Abstract
Gastro-oesophageal reflux and aspiration have been associated with chronic and end-stage lung disease and with allograft injury following lung transplantation. This raises the possibility that bile acids may cause lung injury by damaging airway epithelium. The aim of this study was to investigate the effect of bile acid challenge using the immortalised human bronchial epithelial cell line (BEAS-2B).
The immortalised human bronchial epithelial cell line (BEAS-2B) was cultured. A 48-h challenge evaluated the effect of individual primary and secondary bile acids. Post-challenge concentrations of interleukin (IL)-8, IL-6 and granulocyte−macrophage colony-stimulating factor were measured using commercial ELISA kits. The viability of the BEAS-2B cells was measured using CellTiter-Blue and MTT assays.
Lithocholic acid, deoxycholic acid, chenodeoxycholic acid and cholic acid were successfully used to stimulate cultured BEAS-2B cells at different concentrations. A concentration of lithocholic acid above 10 μmol·L−1 causes cell death, whereas deoxycholic acid, chenodeoxycholic acid and cholic acid above 30 μmol·L−1 was required for cell death. Challenge with bile acids at physiological levels also led to a significant increase in the release of IL-8 and IL6 from BEAS-2B.
Aspiration of bile acids could potentially cause cell damage, cell death and inflammation in vivo. This is relevant to an integrated gastrointestinal and lung physiological paradigm of chronic lung disease, where reflux and aspiration are described in both chronic lung diseases and allograft injury.
Abstract
Bile acid exposure has a significant effect on cytokine production which could contribute to airway injury http://ow.ly/Jwfa309jRqu
Introduction
The airway epithelium plays a critical role in diseased as well as non-diseased lungs, primarily by providing a physical barrier to foreign particles such as particulates and smoke, as well as viruses and bacteria. In addition, it enables a range of pro-inflammatory, anti-microbial and regulatory functions. Epithelial regulatory functions can involve generating and disseminating a range of mediators for various responses, including chemotaxis, inflammatory cell differentiation and activation in both diseased and non-diseased conditions [1].
Many chronic degenerative diseases, including lung allograft dysfunction, are characterised by epithelial damage related to pro-inflammatory cytokines. This process commonly involves bronchial cell injury and necrosis, as well as leukocyte infiltration, leading to inflammatory lung disease [2].
When the lower oesophageal sphincter becomes relaxed, gastric contents may pass into the oesophagus by retrograde flow. Gastro-oesophageal reflux (GOR) is not uncommon and generally may not be harmful [3]. However, certain conditions may lead to GOR becoming pathological, resulting in gastro-oesophageal reflux disease (GORD) [1, 2]. GORD symptoms can be relieved by proton pump inhibitors (PPIs). PPIs work by binding covalently to gastric H+ K+-ATPase pumps, causing irreversible inhibition of the final step in gastric acid secretion. The use of PPIs therefore causes gastric pH to be elevated [4]. PPI treatment can reduce gastric acid secretion, but does not prevent overall gastric aspiration, which includes non-acid components [5]. This is one reason why acid suppression alone may not be entirely effective in preventing GOR [6], and in cases where symptoms are unresponsive to PPI treatment, it is thought that these continued symptoms may be caused by reflux of agents other than gastric acid [7].
Bile acids (BAs) are sparingly water-soluble steroids produced by hepatocytes. Despite being strongly cytotoxic and associated with gastrointestinal malignancy [8], BAs are an essential component of the digestive process, aiding lipid digestion in the small intestine. “Primary BAs” are synthesised by conversion of cholesterol into cholic acid (CA) and chenodeoxycholic acid (CDCA), which are then conjugated to glycine or taurine. These are deconjugated by bacterial enzyme action in the colon into “free BAs”. The action of 7α-dehydroxylase converts free BAs into “secondary BAs” (CDCA into lithocholic acid (LCA) and CA into deoxycholic acid (DCA)).
Gastric mucosa [8, 9], colonic mucosa [9, 10] and hepatocytes [11] are all affected by BAs. However, their toxicity to airway mucosa is less well understood [12]. Patients with a range of airway diseases have been shown to exhibit higher BA concentrations in their bronchoalveolar lavage (BAL) [13]. Gastric aspiration has been revealed to lead to severe pneumonitis [14]. Most therapeutic interventions for GORD and associated inflammatory disease have sought to inhibit acid production by gastric parietal cells. However, a study conducted by Kauer et al. [15] measured both acid (using standard pH tests) and duodenal juice (bilirubin monitoring) reflux in 53 GORD patients. Both types of reflux were found to be present in two-thirds of the patients. Furthermore, higher levels of bilirubin were identified in the refluxate of patients with Barrett's oesophagus. These findings may explain the limited success of PPI therapy in this setting and perhaps why some patients appear more prone to developing Barrett's oesophagus.
The most physiologically relevant cell type to evaluate airways damage is arguably the primary human bronchial epithelial cell (PBEC), but these cells are costly and difficult to culture [16]. Consequently, cell lines have been investigated regarding their ability to provide a useful model to complement the use of PBECs. The human bronchial epithelial cell line, BEAS-2B, for example, is able to secrete interleukin (IL)-6, IL-8 and other cytokines, while maintaining epithelial cell morphology in vitro. These qualities make BEAS-2B a favoured cell line for airway epithelial structure and functional studies [17].
This study hypothesised that BAs, described previously as being present in the lung, can cause damage to the airway epithelium, which may be a mechanism for lung injury. The study aimed to establish a model for airway epithelial cell injury that is relevant to reflux-associated chronic degenerative airways diseases. BEAS-2B cells were challenged with primary and secondary Bas, and pro-inflammatory cytokine levels (IL-8, IL-6 and granulocyte−macrophage colony-stimulating factor (GM-CSF)) were measured by ELISA. These cytokine markers were chosen on the basis of prior studies, which have found these factors to be released by damaged cells, and because they are associated with airway pathophysiology [17].
Materials and methods
Cell culture
BEAS-2B culture
BEAS-2B (obtained from ATCC; LGC Standards, Teddington, UK) is a human bronchial epithelial cell line derived from normal human epithelial cells immortalised using a hybrid of adenovirus 12 and simian virus 40 [18]. BEAS-2B cells were seeded in 24-well plates at a density of 6 to 7×l05 cells·mL−1 and were grown in 500 µL bronchial epithelial growth medium (BEGM) (Lonza, Cambridge, MA, USA) at 5% CO2 and 37°C. This was supplemented with 2 mL bovine pituitary extract (0.004 mL·mL−1), 0.5 mL insulin (5 μg·mL−1), 0.5 mL hydrocortisone (0.5 μg·mL−1), 0.5 mL retinoic acid (0.1 ng·mL−1), 0.5 mL transferrin (10 μg·mL−1), 0.5 mL tri-iodothyronine (6.7 ng·mL−1), 0.5 mL adrenaline (0.5 μg·mL−1), 0.5 mL recombinant epidermal growth factor human (10 ng·mL−1), 100 U·mL−1 l-glutamine and 100 U·mL−1 penicillin/streptomycin (Sigma, Gillingham, UK).
BEAS-2B cell stimulation
At about 80% confluence, BEAS-2B cells were rested for 24 h in serum-free medium (BEBM, 50 mg·mL−1 gentamycin, 50 μg·mL−1 amphotericin B (Lonza), 100 U·mL−1 penicillin, 100 µg·mL−1 streptomycin (Sigma). BAs were dissolved in methanol to prepare stock solutions (100 Mmol·L−1). The solution was diluted with resting medium to achieve a range of experimental concentrations. A total of three experiments with five replicate wells (n=15) were conducted. The following BA concentrations were used: 1, 5, 10, 15, 20, 30, 50, 75 and 100 μmol·L−1. After 48 h incubation, cell culture supernatants were collected to measure cytokine concentrations. Commercial IL-8, IL-6 and GM-CSF ELISA kits were used (R&D Systems, Abingdon, UK).
Cell viability assays
CellTiter-Blue assay
Cell viability was assessed using the CellTiter-Blue assay (Promega, Madison, WI, USA). Known ratios of living to dead cells were used to generate a standard curve (with the dead cell control prepared by exposure to ice-cold methanol for 5 min) and to provide cell mixtures. The standard curve comprised 100% live cells (resting media); 25/75%, 50/50%, 75/25% dead/live cells; and 100% dead cells. This was used to determine the percentages of viable cells. Wavelengths of 560 and 600 nm were used to measure absorbance and determine signal strength.
Thiazolyl blue tetrazolium bromide
The MTT (Sigma, St Louis, MO, USA) assay, which measures mitochondrial function, was used for several experiments. For the MTT assay, cells were cultured and stimulated with BAs. After 48 h, the medium was removed and replaced with 100 µL MTT solution (5 mg·mL−1 in 3 mL of fresh medium without BAs). The plate was incubated for a further 4 h at 37 °C. Formazan crystals developed, and were dissolved in MTT solubilisation solution equal to the original culture medium volume. A gyratory shaker was used to accelerate dissolution. The standard curve comprised 100% live cells (resting media); 25/75%; 50/50%; 75/25% dead/live cells; and 100% dead cells. This was used to determine the percentages of viable cells. Spectrophotometric analysis, with reference and background wavelengths of 570 and 690 nm, was used to measure well absorbance.
Statistical analysis
Analyses were performed using GraphPad Prism v6 for Windows. All data are represented as mean±sem and n indicates the number of repeat experiments performed. For experiments involving comparison of three or more matched groups, repeated measures ANOVA was used followed by the Bonferroni post hoc test, which compares all pairs of groups. In line with convention, p-values of ≤0.05 were considered significant.
Results
Effect of BA on BEAS-2B cell viability
Both the CellTiter-Blue and MTT assays were used to calculate viability. The results revealed no significant difference between the two methods of assessment.
The results showed that cell viability reduced significantly in response to exposure to 5 μmol·L−1 concentrations of LCA (to 78±3%) and then decreased with 10 µmol·L−1 to 46±3%. This decreased further at 20 µmol·L−1 (to 7±3%) (figure 1a).
Cell viability was also reduced significantly by DCA at concentration 50 µmol·L−1 (94±3%). However, 15 and 30 µmol·L−1 DCA did not affect cell viability (figure 1b).
Cell viability also reduced significantly in response to exposure to 50 μmol·L−1 concentrations of CA to 88±3%. This decreased further to the lowest cell viability level at 100 µmol·L−1 (53±3%). The lowest concentration (15 and 30 µmol·L−1) of CA did not affect cell viability (figure 1c).
Cell viability was also diminished significantly by CDCA at concentration 50 µmol·L−1 to 78±3%. The cell viability greatly reduced to 100 µmol·L−1 of CDCA with 23±3%. No difference was found in the cell viability at lowest concentration 15 and 30 µmol·L−1 compared to control (figure 1d).
Release of pro-inflammatory markers by BEAS-2B cells following exposure to BA
Lithocholic acid
IL-8, IL-6 and GM-CSF secretion were all effected in BEAS-2B cells by exposure to lithocholic acid (LCA) at concentrations from 1 to 20 µmol·L−1. IL-8 levels increased significantly at 5 µmol·L−1 and increased further up to the maximum level at 10 µmol·L−1. The level of IL-8 production then fell off at 20 µmol·L−1 but was still significantly higher than the control (figure 2a). Moreover, there was a significant increase in IL-6 levels with LCA at concentrations of 10, 15 and 20 μmol·L−1, but not at 1 and 5 µmol·L−1. The result revealed that the highest level of IL-6 production was at 10 µmol·L−1 and then declined as the concentration of LCA increased to 20 µmol·L−1 (figure 2b).The GM-CSF levels increased significantly at 5 µmol·L−1 LCA, and rose further to the maximum level at 10 µmol·L−1. The level of GM-CSF production then fell off at 20 µmol·L−1 (figure 2c).
Deoxycholic acid
Cells were treated with deoxycholic acid (DCA) at concentrations from 15 to 100 µmol·L−1. IL-8 levels increased significantly at 30 µmol·L−1 and rose further to the maximum level at 75 µmol·L−1. The level of IL-8 production than fell off at 100 µmol·L−1 but was still significantly higher than the control. However, no significant increase was observed using concentrations of 15 µmol·L−1 DCA (figure 3a). IL-6 levels rose considerably with DCA at concentrations of 50, 75 and 100 μmol·L−1, but not at 15 and 30 µmol·L−1. The highest level of IL-6 secretion was with 100 µmol·L−1. The levels reduced to 50 µmol·L−1 but remained significantly above the control (figure 3b). Furthermore, the GM-CSF levels increased significantly at 30 µmol·L−1 and increased further up to the maximum level at 75 µmol·L−1. The level of GM-CSF production then decreased at 100 µmol·L−1 but remained significantly higher than the control. However, no significant increase was observed using a concentration of 15 µmol·L−1 DCA (figure 3c).
Cholic acid
ELISA analysis detected IL-8, IL-6 and GM-CSF in cell culture supernatants following cholic acid (CA) stimulation of BEAS-2B. IL-8 levels increased significantly with CA at concentrations of 50, 75 and 100 μmol·L−1, but not at 15 and 30 µmol·L−1 (figure 4a). The highest level of IL-8 secretion was with 100 µmol·L−1. The levels reduced to 50 µmol·L−1 but remained considerably above the control. All CA concentrations significantly elevated IL-6 levels except 15 μmol·L−1. IL-6 production increased in a dose-dependent manner (figure 4b). Furthermore, the GM-CSF levels increased significantly at 30 µmol·L−1 and rose further to the maximum level at 75 µmol·L−1. The level of GM-CSF production then dropped at 100 µmol·L−1 but was still significantly higher than the control (figure 4c).
Chenodeoxycholic acid
Concentrations of chenodeoxycholic acid (CDCA) from 15 to 100 µmol·L−1 were tested. IL-8 levels increased significantly, but this was not evident at concentrations of 15 µmol·L−1 (figure 5a). It is clear that IL-8 levels increased significantly at 30 µmol·L−1 and rose further to the maximum level at 50 µmol·L−1. The level of IL-8 production than fell off at 100 µmol·L−1 but was still significantly higher than the control. The IL-6 levels were increased with CDCA at concentrations of 30, 50, 75 and 100 μmol·L−1, but not at 15 µmol·L−1 (figure 5b). The highest level of IL-6 secretion was with 100 µmol·L−1. The levels reduced at 30 µmol·L−1 but remained significantly above the control. ELISA detected GM-CSF following CDCA stimulation of BEAS-2B cells. The CDCA significantly upregulated GM-CSF production at 30, 50, 75 and 100 μmol·L−1 (figure 5c). It was apparent that GM-CSF production increased significantly at 30 µmol·L−1 and rose further to a maximum level at 50 µmol·L−1. The level of GM-CSF production then decreased at 100 µmol·L−1 but was still significantly higher than the control.
Discussion
Diseases of the lung are a major source of mortality and morbidity worldwide and are becoming increasingly prevalent. Chronic obstructive pulmonary disease (COPD), for example, is the fifth biggest cause of death globally, while cystic fibrosis (CF) and interstitial lung disease are not rare and have a poor prognosis, including low quality of life and premature death. A connection between lung disease and GORD has long been suspected [15, 19] and high incidences of GORD are known to affect sufferers of idiopathic pulmonary fibrosis, asthma, CF and COPD [20, 21]. It is of interest that in COPD it has been revealed that the second most powerful predictor of exacerbations is a history of heartburn, a symptom associated with GOR [22]. Refluxate from GORD patients has been found to contain BA levels up to a millimolar level [23]. We have previously described high levels of BAs in the lungs of people with CF, both before and after transplantation [13].
The potential role of BAs in respiratory infection and inflammation is becoming increasingly recognised, although the mechanisms are still not well understood. Most BAs in humans exist in their conjugated forms [24]. Treatment for GORD, including the use of PPIs and acid suppression therapies can result in deconjugation of BAs [25], producing a higher ratio of deconjugated to conjugated BAs in patients undergoing GORD treatment [25]. CDCA, DCA and LCA have all been found in their unconjugated forms in airway secretions [26], so their effect may be of particular relevance to patients with GORD.
The effects of both primary and secondary BAs on BEAS-2B viability were studied. Cell viability was found to be affected depending on BA concentration. There was an inverse relationship between BA concentration and measured cell viability, with increasing concentration and decreasing cell viability in a dose-dependent manner. LCA at 5 μmol·L−1 caused a decrease in cell viability and 20 μmol·L−1 killed nearly all cells. These results are supported by data provided by Wu et al. [18], whose study exposed human epithelial cells to BAs. The precise mechanism by which BA exposure affects the viability of BEAS-2B cells remains unclear. It is known that cell membranes and cationic permeability are disrupted by BAs in type II pneumocytes in vitro [27], which may provide a mechanistic explanation for our study findings. In vivo, it is possible that this detergent effect may cause mucosal barrier breakdown, disrupting the mucus layer and surfactant function in the lungs, consequently damaging type II pneumocytes, which produce both surfactant protein and phospholipids [27, 28].
BEAS-2B cells retain the morphology of an epithelial cell in vitro [18] and release IL-8 and IL-6 in addition to other cytokines [29]. Under submerged conditions, BEAS-2B cells are commonly used as the in vitro model of human airways epithelium, especially within studies on inhalation toxicity.
Our work demonstrates that cell viability decreased depending on BA concentration. There was a dose response to BA concentration, where increasing levels of BAs were associated with decreased cell viability. Our data demonstrated that low levels of LCA (5 μmol·L−1) affected viability and inflammatory cytokine outputs from human bronchial epithelial cells. Moreover, LCA effects occurred at five- to 10-fold lower concentrations compared to the other BAs.
Alongside the potential effect on cell viability, our data show that BA stimulation of epithelial cells is associated with a release of pro-inflammatory cytokines. The study found an increase in the expression of IL-8, IL-6 and GM-CSF following BA challenge.
The lung diseases in which reflux and aspiration are implicated all involve a contribution from acute or chronic inflammation, leading frequently to the use of anti-inflammatory medication, such as steroids [17]. The data gathered in this study support, overall, the hypothesis that BA exposure may have a significant effect on cytokine production and subsequently contribute to airway injury.
IL-8 is a neutrophilic chemoattractant and an angiogenic factor. Immunostaining has been used to identify IL-8 localisation in peribronchial lesions [30]. IL-6 plays a role in the activation of monocytes, T-cell differentiation and B-cell maturation. Moreover, it serves as a pyrogen, inducing fever in response to infection [31]. GM-CSF is involved in macrophage activation and is a strong chemoattractant for neutrophils. It stimulates microbicidal and phagocytotic activity, oxidative metabolism, and improves the cytotoxicity of both macrophages [32] and neutrophils [33]. Increased levels of IL-8, IL-6 and GM-CSF are a common finding in airways and lung disease where reflux and aspiration have been implicated as a possible injury [17, 31, 34].
A strength of our study, which is complementary to the limited amount of previous work available, was that the use of BEAS-2B allowed a comprehensive evaluation of a range of primary and secondary BAs, cytotoxicity and inflammatory cytokine production. This was possible because the available cell numbers did not limit the experiments.
Our work could be strengthened by studies involving PBECs. In this study, submerged culture systems were used to examine the association between airways diseases and BAs. Future work could include establishing PBECs at an air–liquid interface, where cells are differentiated into ciliated epithelial cells. The present BA stimulation could then usefully be repeated in in differntiated cells.
Conclusions
Our model data, revealing cytotoxicity and increased levels of inflammatory cytokines associated with BA stimulation of BEAS-2B epithelial cells, may explain the decreased lung function and chronic inflammation connected with aspiration in respiratory disease patients. These findings and further studies may contribute to an integrated “aerodigestive paradigm” regarding the understanding of reflux-related chronic lung diseases. Increased knowledge in this field may represent an opportunity to consider new approaches to patient therapy.
Footnotes
Conflict of interest: None declared.
- Received September 29, 2016.
- Accepted February 3, 2016.
- Copyright ©ERS 2017
This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.