RT Journal Article
SR Electronic
T1 Trans-basement membrane migration of eosinophils induced by LPS-stimulated neutrophils from human peripheral blood <em>in vitro</em>
JF ERJ Open Research
JO erjor
FD European Respiratory Society
SP 00003-2015
DO 10.1183/23120541.00003-2015
VO 1
IS 2
A1 Fuyumi Nishihara
A1 Kazuyuki Nakagome
A1 Takehito Kobayashi
A1 Toru Noguchi
A1 Ryuichiro Araki
A1 Yoshitaka Uchida
A1 Tomoyuki Soma
A1 Makoto Nagata
YR 2015
UL http://openres.ersjournals.com/content/1/2/00003-2015.abstract
AB In the airways of severe asthmatics, an increase of neutrophils and eosinophils is often observed despite high-dose corticosteroid therapy. We previously reported that interleukin-8-stimulated neutrophils induced trans-basement membrane migration (TBM) of eosinophils, suggesting the link between neutrophils and eosinophils. Concentrations of lipopolysaccharide (LPS) in the airway increase in severe asthma. As neutrophils express Toll-like receptor (TLR)4 and can release chemoattractants for eosinophils, we investigated whether LPS-stimulated neutrophils modify eosinophil TBM.Neutrophils and eosinophils were isolated from peripheral blood of healthy volunteers and severe asthmatics. Eosinophil TBM was examined using a modified Boyden's chamber technique. Eosinophils were added to the upper compartment, and neutrophils and LPS were added to the lower compartment. Migrated eosinophils were measured by eosinophil peroxidase assays.LPS-stimulated neutrophils induced eosinophil TBM (about 10-fold increase), although LPS or neutrophils alone did not. A leukotriene B4 receptor antagonist, a platelet-activating factor receptor antagonist or an anti-TLR4 antibody decreased eosinophil TBM enhanced by LPS-stimulated neutrophils by almost half. Neutrophils from severe asthmatics induced eosinophil TBM and lower concentrations of LPS augmented neutrophil-induced eosinophil TBM.These results suggest that the combination of neutrophils and LPS leads eosinophils to accumulate in the airways, possibly involved the pathogenesis of severe asthma.LPS-stimulated neutrophils induced eosinophil TBM, which may be involved in the pathogenesis of severe asthma http://ow.ly/UR4jP