RT Journal Article
SR Electronic
T1 The inflammasome pathway in stable COPD and acute exacerbations
JF ERJ Open Research
JO erjor
FD European Respiratory Society
SP 00002-2016
DO 10.1183/23120541.00002-2016
VO 2
IS 3
A1 Rosa Faner
A1 Patricia Sobradillo
A1 Aina Noguera
A1 Cristina Gomez
A1 Tamara Cruz
A1 Alejandra López-Giraldo
A1 Eugeni Ballester
A1 Nestor Soler
A1 Juan I. Arostegui
A1 Pablo Pelegrín
A1 Roberto Rodriguez-Roisin
A1 Jordi Yagüe
A1 Borja G. Cosio
A1 Manel Juan
A1 Alvar Agustí
YR 2016
UL http://openres.ersjournals.com/content/2/3/00002-2016.abstract
AB Chronic obstructive pulmonary disease (COPD) is characterised by pulmonary and systemic inflammation that bursts during exacerbations of the disease (ECOPD). The NLRP3 inflammasome is a key regulatory molecule of the inflammatory response. Its role in COPD is unclear.We investigated the NLRP3 inflammasome status in: 1) lung tissue samples from 38 patients with stable COPD, 15 smokers with normal spirometry and 14 never-smokers; and 2) sputum and plasma samples from 56 ECOPD patients, of whom 41 could be reassessed at clinical recovery.We observed that: 1) in lung tissue samples of stable COPD patients, NLRP3 and interleukin (IL)-1β mRNA were upregulated, but both caspase-1 and ASC were mostly in inactive form, and 2) during infectious ECOPD, caspase-1, oligomeric ASC and associated cytokines (IL-1β, IL-18) were significantly increased in sputum compared with clinical recovery.The NLRP3 inflammasome is primed, but not activated, in the lungs of clinically stable COPD patients. Inflammasome activation occurs during infectious ECOPD. The results of this study suggest that the inflammasome participates in the inflammatory burst of infectious ECOPD.The NLRP3 inflammasome is primed in stable COPD lungs, then activated during infectious exacerbation http://ow.ly/Wopi300DXcT