RT Journal Article
SR Electronic
T1 Two weeks of intermittent hypoxic exposure induce lipolysis at the fat tissue level in healthy human subjects
JF ERJ Open Research
JO erjor
FD European Respiratory Society
SP P78
DO 10.1183/23120541.sleepandbreathing-2017.P78
VO 3
IS suppl 1
A1 Marie Netchitaïlo
A1 Fanny Fabre
A1 Anne Briançon-Marjollet
A1 Elise Belaidi-Corsat
A1 Claire Arnaud
A1 Anne-Laure Borel
A1 Patrick Lévy
A1 Jean-Louis Pépin
A1 Renaud Tamisier
YR 2017
UL http://openres.ersjournals.com/content/3/suppl_1/P78.abstract
AB Introduction Associated cardiovascular morbidity in Obstructive Sleep Apnea Syndrome is mainly caused by chronic intermittent hypoxia (CIH) stimulus originating sympathetic hyperactivity. In rodent exposed to CIH, the adipose tissue is the host of local inflammation and metabolism alterations. Our objective was to assess the impact of increased sympathetic activity induced by 14 nights of CIH exposure on Glucose control and lipid profile and subcutaneous fat tissue in non-obese healthy humans.Methods In this prospective, single-blinded cross-over study, 12 healthy subjects underwent 2 phases of exposure of 14 nights randomized to CIHvs.air. Sympathetic activity was measured by peroneal microneurography. Fasting glucose, insulin, c-peptide and FFA were assessed at rest and during a multisampling oral glucose tolerance test. Subcutaneous adipose tissue biopsies were obtained after each phase of exposure. We assessed morphological and functional changes of the adipose tissue using immuno-histology and RT-PCR.Results After two weeks exposures of CIH versus room air, sympathetic activity significantly increased (p<0.05). Although insulin sensitivity seemed not to change significantly, fasting FFA increased. At the fat tissue level, IH induced shrunken adipocytes (3.3 vs. 2.7 x103μm2; p=0.03), increased lipolysis (Hormon Sensitive Lipase expression x 2.95; p=0.02) and increased lipogenesis (Fatty Acid Synthase expression x 3.62; p=0.04). No difference was found in markers of inflammation in the subcutaneous adipose tissue.Conclusion In this experimental setting of high sympathetic tone, CIH induced an increase in lipolysis that may be partly responsible for associated cardio metabolic consequences.