RT Journal Article
SR Electronic
T1 Protected sampling is preferable in bronchoscopic studies of the airway microbiome
JF ERJ Open Research
JO erjor
FD European Respiratory Society
SP 00019-2017
DO 10.1183/23120541.00019-2017
VO 3
IS 3
A1 Rune Grønseth
A1 Christine Drengenes
A1 Harald G. Wiker
A1 Solveig Tangedal
A1 Yaxin Xue
A1 Gunnar Reksten Husebø
A1 Øistein Svanes
A1 Sverre Lehmann
A1 Marit Aardal
A1 Tuyen Hoang
A1 Tharmini Kalananthan
A1 Einar Marius Hjellestad Martinsen
A1 Elise Orvedal Leiten
A1 Marianne Aanerud
A1 Eli Nordeide
A1 Ingvild Haaland
A1 Inge Jonassen
A1 Per Bakke
A1 Tomas Eagan
YR 2017
UL http://openres.ersjournals.com/content/3/3/00019-2017.abstract
AB The aim was to evaluate susceptibility of oropharyngeal contamination with various bronchoscopic sampling techniques.67 patients with obstructive lung disease and 58 control subjects underwent bronchoscopy with small-volume lavage (SVL) through the working channel, protected bronchoalveolar lavage (PBAL) and bilateral protected specimen brush (PSB) sampling. Subjects also provided an oral wash (OW) sample, and negative control samples were gathered for each bronchoscopy procedure. DNA encoding bacterial 16S ribosomal RNA was sequenced and bioinformatically processed to cluster into operational taxonomic units (OTU), assign taxonomy and obtain measures of diversity.The proportion of Proteobacteria increased, whereas Firmicutes diminished in the order OW, SVL, PBAL, PSB (p<0.01). The alpha-diversity decreased in the same order (p<0.01). Also, beta-diversity varied by sampling method (p<0.01), and visualisation of principal coordinates analyses indicated that differences in diversity were smaller between OW and SVL and OW and PBAL samples than for OW and the PSB samples. The order of sampling (left versus right first) did not influence alpha- or beta-diversity for PSB samples.Studies of the airway microbiota need to address the potential for oropharyngeal contamination, and protected sampling might represent an acceptable measure to minimise this problem.Protected bronchoscopic sampling is most suitable for identification of a distinct airway microbiome http://ow.ly/qIIy30eqB9M