TABLE 2

PD-1 on T-cell subsets

Patient	PD-1⁺ aT_reg			PD-1⁺ CD8⁺			PD-1⁺ actCD4⁺			PD-1^hi actCD4⁺			PD-1⁺ naïve CD4⁺			PD-1⁺ cytCD4⁺
Patient	PBMC	TDLN	NTDLN	PBMC	TDLN	NTDLN	PBMC	TDLN	NTDLN	PBMC	TDLN	NTDLN	PBMC	TDLN	NTDLN	PBMC	TDLN	NTDLN
1	31	–	–	10	29	27	28	61	44	0.02	6.7	1.2	5	44	14	36	64	53
2	30	98		32	81		23	50		0.34	8.3		2	5		26	87
3	44	77	–	48	74	64	50	45	–	0.55	3.9	–	63	3	–	57	61	–
4	32		52	43		45	43		55	0.04		3.4	9		6	34		42
5	28	–	56	21	52	13	23	41	32	0.15	2.4	0.7	0.4	0.9	1	30	53	44
6	11		35	30		49	21		33	0.28		1.8	15		29	6		10
7	2	–		8	40		3	15		0.17	1.4		0.3	2		3	13
8	24	83/–		31	85/87		23	55/62		0.06	4.9/11.7		4	13/4		22	69/82
9	3	15		19	43		9	23		0.08	1.0		4	3		4	11
10	5	48		10	37		8	22		0.35	3.4		0.8	6		4	17
11	6	38		7	26		14	26		0.15	5.6		4	3		11	28

Data are presented as percentages of PD-1⁺ cells gated for the different T-cell subsets per patient. Patients 4 and 6 were excluded from the comparative analyses because no tumour-draining lymph node (TDLN) sample was available. Specimens that were not available for research are left open. Bold rows represent those patients who died of the disease within the monitoring period. aT_reg: activated regulatory T-cell; act: activated; cyt: cytokine-secreting; PBMC: peripheral blood mononuclear cell; NTDLN: non-tumour draining lymph node; −: could not be evaluated due to too low counts.