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Suppressive effects of sivelestat on interleukin 8 and TNF-α production from LPS-stimulated granulocytes in whole blood culture

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Abstract

Purpose

The goal of the study was to examine the effects of sivelestat sodium hydrate (sivelestat), a neutrophil elastase inhibitor, on production of cytokines in granulocytes and monocytes, using flow cytometry after cytokine staining in whole blood culture.

Methods

Blood samples were collected from healthy volunteers. Vehicle (control group), lipopolysaccharide (LPS) (LPS group), or LPS + sivelestat (sivelestat group) were added to the whole blood, followed by addition of a protein transport inhibitor in each group. After incubation, staining for cytokines retained in the cells was performed by addition of an anti-interleukin 8 (IL-8) or anti-tumor necrosis factor-α (TNF-α) antibody. The cells were then analyzed using flow cytometry.

Results

Granulocytic production of IL-8 induced by 1 ng/ml LPS was significantly (P < 0.05) inhibited by treatment with 1 μg/ml sivelestat, and upregulation of IL-8 by 10 ng/ml LPS was also significantly (P < 0.05) suppressed by 1 and 10 μg/ml sivelestat. Addition of 10 or 100 μg/ml sivelestat significantly (P < 0.05) inhibited the production of TNF-α from granulocytes induced by 10 ng/ml LPS. Sivelestat did not significantly inhibit LPS-induced monocytic production of TNF-α and IL-8.

Conclusion

Suppression of granulocytic production of IL-8 and TNF-α by sivelestat suggests that this drug may be useful for treatment of morbid conditions involving IL-8 and TNF-α at onset.

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Acknowledgments

We thank Ms. Kayo Kishi for technical assistance.

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Authors and Affiliations

  1. Department of Critical Care Medicine, Iwate Medical University, 19-1 Uchimaru, Morioka, 020-8505, Japan

    Shigehiro Shibata, Gaku Takahashi, Nobuki Shioya, Katsuya Inada & Shigeatsu Endo

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  1. Shigehiro Shibata
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  2. Gaku Takahashi
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  3. Nobuki Shioya
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  4. Katsuya Inada
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  5. Shigeatsu Endo
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Correspondence to Shigehiro Shibata.

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Shibata, S., Takahashi, G., Shioya, N. et al. Suppressive effects of sivelestat on interleukin 8 and TNF-α production from LPS-stimulated granulocytes in whole blood culture. J Anesth 24, 901–907 (2010). https://doi.org/10.1007/s00540-010-1030-2

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  • DOI: https://doi.org/10.1007/s00540-010-1030-2

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