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Immunohistochemical distribution of SP-D, compared with that of SP-A and KL-6, in interstitial pneumonias

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Abstract

The immunohistochemical distribution of SP-D was compared with that of SP-A and KL-6 using a monoclonal antibody in lung tissues of 15 cases of collagen vascular disease-associated interstitial pneumonia (CVD-IP), 4 cases of hypersensitivity pneumonitis (CHP), and 6 cases of other diseases to determine their differences in distribution. In this study, the main targets were alveolar epithelial cells, especially those in the regenerating stage, as well as lymph vessels and stroma. The cytoplasm of type II alveolar epithelial cells and Clara cells was positive for SP-D, with sharp margins; interestingly, however, during the process of regeneration large positive cells were intermingled with relatively small negative cells, even in the same row of cells. In sharp contrast, staining for SP-A and KL-6 was positive in the cytoplasm of all the regenerating alveolar epithelial cells, as well as Clara cells. Staining for KL-6 was usually positive in the surface of air spaces in linear fashion. Staining for SP-A was also positive in elastic fibers in vascular walls. In areas of destruction of pulmonary structures, loose stroma and the endothelial cells of lymph vessels as well as their contents were distinctly positive for SP-A and/or KL-6 but not SP-D. Judging from these results in pulmonary tissues of CVD-IP and HP, SP-D might be a marker for maturity of regenerating epithelial cells. Both SP-A and KL-6 were detected in intimate relationship to the stage of regeneration of alveolar epithelial cells and were expressed before SP-D. In addition, the lymph vessels play a very important role in transfer of KL-6 into the bloodstream.

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Authors and Affiliations

  1. Division of Pathology, Matsuyama-shimin Hospital, Matsuyama, Ehime, 790-0067, Japan

    Yuji Ohtsuki

  2. Department of Internal Medicine, Ehime Prefectural Central Hospital, Ehime, Japan

    Norihiko Nakanishi & Nobuo Ueda

  3. Department of Medicine and Therapeutics, Control and Prevention of Infectious Diseases, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

    Jiro Fujita

  4. Department of Internal Medicine, Toyokawa Municipal Hospital, Aichi, Japan

    Takeo Yoshinouchi

  5. Department of Hematology and Respiratory Medicine, Kochi Medical School, Kochi University, Kochi, Japan

    Makoto Kobayashi

  6. Department of Pathology, Kochi Medical School, Kochi University, Kochi, Japan

    Gang-Hong Lee & Mutsuo Furihata

Authors
  1. Yuji Ohtsuki
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  2. Norihiko Nakanishi
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  3. Jiro Fujita
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  4. Takeo Yoshinouchi
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  5. Makoto Kobayashi
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  6. Nobuo Ueda
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  7. Gang-Hong Lee
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  8. Mutsuo Furihata
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Correspondence to Yuji Ohtsuki.

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Ohtsuki, Y., Nakanishi, N., Fujita, J. et al. Immunohistochemical distribution of SP-D, compared with that of SP-A and KL-6, in interstitial pneumonias. Med Mol Morphol 40, 163–167 (2007). https://doi.org/10.1007/s00795-007-0360-0

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  • DOI: https://doi.org/10.1007/s00795-007-0360-0

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