Human bronchial epithelial cells with integrated SV40 virus T antigen genes retain the ability to undergo squamous differentiation

doi:10.1111/j.1432-0436.1988.tb00592.x

Differentiation

Volume 38, Issue 1, June 1988, Pages 60-66

https://doi.org/10.1111/j.1432-0436.1988.tb00592.x Get rights and content

Summary

Human bronchial epithelial cells transformed by either DNA virus infection (SV40 or Adenovirus 12-SV40 hybrid virus) or transfection with the SV40 large T antigen gene were studied for their ability to undergo squamous differentiation when exposed to 12-O-tetradecanoylphorbol-13 acetate (TPA), transforming growth factor-β₁ (TGF-β₁), or fetal bovine serum (FBS), agents that induce the squamous differentiation of normal human bronchial epithelial cells. Squamous differentiation occurred in all ten T-antigen-positive cell cultures when they were exposed to either FBS or TGF-β₁, but none differentiated when exposed to TPA. From one cell line, designated BEAS-2B, two subclones were isolated, one of which was induced to undergo squamous differentiation by FBS, and a second that failed to undergo squamous differentiation and was mitogenically stimulated when exposed to serum. These phenotypically different subclones provide a new in vitro cellular system for delineating the mechanism(s) of human bronchial epithelial cell squamous differentiation in response to FBS or TGF-β₁

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Human bronchial epithelial cells with integrated SV40 virus T antigen genes retain the ability to undergo squamous differentiation

Summary

Biochim Biophys Acta

Cell

Exp Cell Res

Differentiation

Exp Cell Res

J Invest Derm

Exp Cell Res

Lancet

Cell

Hum Pathol

Biochim Biophys Acta

Transforming growth factors in nonncoplastic tissues and their role in controlling cell growth

Differentiation of human epidermal cells transformed by SV40

J Cell Biol

Long-term organ culture of human bronchial epithelium

Cancer Res

Human placental cells transformed by tsA mutants of simian virus 40: A model system for the study of placental functions

Proc Natl Acad Sci USA

Establishment of clonal human placental cells synthesizing human choriogonadotropin

Proc Natl Acad Sci USA

Transformation by simian virus 40 of spleen cells from a hyper-immune rabbit: evidence for synthesis of immunoglobulin by the transformed cells

Proc Natl Acad Sci USA

Parathyroid hormone production in vitro by human parathyroid cells transformed by simian virus 40

Science

Respiratory carcinogenesis and cancer epidemiology

Human tissues and cells in carcinogenesis research

Cancer Res

Subpopulations of human bronchial epithelial cells in culture respond heterogeneously to 12-O-tetradecanoylphor-bol-12-acetate (TPA) and other modulations of differentiation

Carcinogenesis

Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters

J Biol Chem

Classification and pathological anatomy of lung cancer