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Effects of bile acids on human airway epithelial cells: implications for aerodigestive diseases

Adil Aldhahrani, Bernard Verdon, Chris Ward, Jeffery Pearson
ERJ Open Research 2017 3: 00107-2016; DOI: 10.1183/23120541.00107-2016
Adil Aldhahrani
1Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
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Bernard Verdon
1Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
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Chris Ward
1Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
2Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK
3These authors contributed equally
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  • For correspondence: chris.ward@ncl.ac.uk
Jeffery Pearson
1Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK
3These authors contributed equally
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  • FIGURE 1
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    FIGURE 1

    Effects of BAs on BEAS-2B cell viability. BEAS-2B cell viability following stimulation with a) lithocholic, b) deoxycholic, c) cholic and d) chenodeoxycholic acids at varying concentrations. Viability is measured as a percentage of vehicle control after 48 h exposure. Data are presented as mean±sem. Negative controls were measured by fixing the cells in methanol before adding the TiterBlue and MTT reagent (n=6). C: control; CTB: CellTiter-Blue assay; MTT: thiazolyl blue tetrazolium bromide. **: p=0.01; ****: p<0.0001 versus controls.

  • FIGURE 2
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    FIGURE 2

    Measurement of a) L-8, b) IL-6 and c) GM-CSF release by BEAS-2B cells stimulated with lithocholic acid for 48 h. Data are presented as mean±sem (n=6). ELISA measured cytokine secretion in cell supernatants. Statistical analysis was performed using one-way ANOVA test. p-values of 0.05 or less were regarded as significant. C: control; IL: interleukin; GM-CSF: granulocyte−macrophage colony-stimulating factor.

  • FIGURE 3
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    FIGURE 3

    Measurement of a) IL-8, b) IL-6 and c) GM-CSF release by BEAS-2B cells stimulated with deoxycholic acid for 48 h. Data are presented as mean±sem (n=6). ELISA measured cytokine secretion in cell supernatants. Statistical analysis was performed using one-way ANOVA test. p-values of 0.05 or less were regarded as significant. C: control; IL: interleukin; GM-CSF: granulocyte−macrophage colony-stimulating factor.

  • FIGURE 4
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    FIGURE 4

    Measurement of a) IL-8, b) IL-6 and c) GM-CSF release by BEAS-2B cells stimulated with cholic acid for 48 h. Data are presented as mean±sem (n=6). ELISA measured cytokine secretion in cell supernatants. Statistical analysis was performed using one-way ANOVA test. p-values of 0.05 or less were regarded as significant. C: control; IL: interleukin; GM-CSF: granulocyte−macrophage colony-stimulating factor.

  • FIGURE 5
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    FIGURE 5

    Measurement of a) IL-8, b) IL-6 and c) GM-CSF release by BEAS-2B cells stimulated with chenodeoxycholic acid for 48 h. Data are presented as mean±sem (n=6). ELISA measured cytokine secretion in cell supernates. Statistical analysis was performed using one-way ANOVA test. p-values of 0.05 or less were regarded as significant. C: control; IL: interleukin; GM-CSF: granulocyte−macrophage colony-stimulating factor.

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Effects of bile acids on human airway epithelial cells: implications for aerodigestive diseases
Adil Aldhahrani, Bernard Verdon, Chris Ward, Jeffery Pearson
ERJ Open Research Jan 2017, 3 (1) 00107-2016; DOI: 10.1183/23120541.00107-2016

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Effects of bile acids on human airway epithelial cells: implications for aerodigestive diseases
Adil Aldhahrani, Bernard Verdon, Chris Ward, Jeffery Pearson
ERJ Open Research Jan 2017, 3 (1) 00107-2016; DOI: 10.1183/23120541.00107-2016
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