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“Trans-basement membrane migration of eosinophils induced by LPS-stimulated neutrophils from human peripheral blood in vitro” Fuyumi Nishihara, Kazuyuki Nakagome, Takehito Kobayashi, Toru Noguchi, Ryuichiro Araki, Yoshitaka Uchida, Tomoyuki Soma and Makoto Nagata. ERJ Open Res 2015; 1: 00003-2015.

ERJ Open Research 2017 3: 00003-2015-AUT; DOI: 10.1183/23120541.50003-2017
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This article was originally published with incorrect p-values in figures 2 and 6. The revised figures are shown below and have been corrected in the article itself. The authors apologise for this error.

FIGURE 2
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FIGURE 2

Dose-dependent effect of lipopolysaccharide (LPS) on neutrophil-induced trans-basement membrane migration of eosinophils in healthy volunteers. Neutrophils (2×104 cells) were stimulated with various concentrations of LPS (0.01–100 µg·mL−1) and then placed into the lower compartment. Eosinophils (1×105 cells) were added to the upper compartment of a chamber with a Matrigel-coated Transwell insert. After 120 min of incubation, migrated eosinophils in the lower chamber were measured by eosinophil peroxidase assays (n=4). Data are presented as mean±sem. **: p<0.01 versus spontaneous migration (0 µg·mL−1 LPS) by Tukey test; ***: p<0.001 versus spontaneous migration (0 µg·mL−1 LPS) by Tukey test.

FIGURE 6
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FIGURE 6

Dose-dependent effect of lipopolysaccharide (LPS) on neutrophil-induced trans-basement membrane migration of eosinophils in severe asthmatics. Neutrophils (2×104 cells) from severe asthmatics were stimulated with various concentrations of LPS (0.01–100 µg·mL−1), and then placed into the lower compartment of a chamber with a Matrigel-coated Transwell insert. Eosinophils (1×105 cells) from severe asthmatics were added to the upper compartment. After 120 min of incubation, migrated eosinophils in the lower chamber were measured by eosinophil peroxidase assays (n=4). Data are presented as mean±sem. *: p<0.05 versus spontaneous migration (0 µg·mL−1 LPS) by Tukey test; **: p<0.01 versus spontaneous migration (0 µg·mL−1 LPS) by Tukey test.

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This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.

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“Trans-basement membrane migration of eosinophils induced by LPS-stimulated neutrophils from human peripheral blood in vitro” Fuyumi Nishihara, Kazuyuki Nakagome, Takehito Kobayashi, Toru Noguchi, Ryuichiro Araki, Yoshitaka Uchida, Tomoyuki Soma and Mako…
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“Trans-basement membrane migration of eosinophils induced by LPS-stimulated neutrophils from human peripheral blood in vitro” Fuyumi Nishihara, Kazuyuki Nakagome, Takehito Kobayashi, Toru Noguchi, Ryuichiro Araki, Yoshitaka Uchida, Tomoyuki Soma and Makoto Nagata. ERJ Open Res 2015; 1: 00003-2015.
ERJ Open Research Jul 2017, 3 (3) 00003-2015-AUT; DOI: 10.1183/23120541.50003-2017

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“Trans-basement membrane migration of eosinophils induced by LPS-stimulated neutrophils from human peripheral blood in vitro” Fuyumi Nishihara, Kazuyuki Nakagome, Takehito Kobayashi, Toru Noguchi, Ryuichiro Araki, Yoshitaka Uchida, Tomoyuki Soma and Makoto Nagata. ERJ Open Res 2015; 1: 00003-2015.
ERJ Open Research Jul 2017, 3 (3) 00003-2015-AUT; DOI: 10.1183/23120541.50003-2017
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