Figures
- FIGURE 1
Mean taxonomic distribution at the phylum level, by sampling method, for all 125 individuals (unrarefied). OW: oral wash; SVL: small-volume lavage in the left upper lobe; BAL1: first fraction of protected bronchoalveolar lavage (BAL) from right middle lobe; BAL2: second fraction of protected BAL from right middle lobe; PSB: protected specimen brush from right lower lobe and left upper lobe. No legend for smallest phylae.
- FIGURE 2
Box-plot of alpha-diversity measured by wholetree phylogenetic differences grouped according to sampling method and chronic obstructive pulmonary disease (COPD) status. Rarefied at 1000 sequences. OW: oral wash sample; SVL: small-volume lavage from left upper lobe; PBAL1: first fraction of protected bronchoalveolar lavage (BAL); PBAL2: second fraction of protected BAL; right PSB: protected specimen brush from right lower lobe; left PSB: protected specimen brush from left upper lobe.
- FIGURE 3
Principal coordinates analyses on unweighted UniFrac distance matrix comparing sampling methods in the MicroCOPD to oral wash samples. Rarefied at 1000 sequences. LUL: left upper lobe; BAL: bronchoalveolar lavage; RML: right middle lobe; RLL: right lower lobe.
Tables
- TABLE 1
Characteristics of 125 subjects of the MicroCOPD study
COPD Asthma Control Subjects 64 3 58 Males 34 (53.1%) 2 (67.7%) 34 (58.6%) Current smokers 15 (23.4%) 0 16 (27.6%) Ex-smokers 48 (75.0%) 2 (67.7%) 35 (60.3%) Never-smokers 1 (1.6%) 1 (33.3%) 7 (12.1%) Smoking exposure pack-years 28.49±16.08 20.88±24.22 22.83±18.55 FEV1 % predicted 56.83±16.30 88.31±11.37 100.71±11.00 Age years 68.73±7.23 64.41±9.1 64.89±8.43 Use of inhaled corticosteroids 44 (68.8%) 1 (33.3%) 1 (1.7%) Data are presented as mean±sd unless otherwise stated. COPD: chronic obstructive pulmonary disease; FEV1: forced expiratory volume in 1 s.
Supplementary Material
Please note: supplementary material is not edited by the Editorial Office, and is uploaded as it has been supplied by the author.
Supplementary material and methods 00019-2017_supp
Illustration of protected bronchoalveolar lavage (PBAL) with phosphate buffered saline (PBS). 00019-2017_figureS1
Illustration of protected specimen brush (PSB) sampling. 00019-2017_figureS2
Box-plot of alpha-diversity measured by wholetree phylogenetic differences by which lung that was sampled first and right/left protected specimen brush (PSB). Rarefied at 1000 sequences. 00019-2017_figureS3
Principal coordinates analyses of unweighted UniFrac distances by which lung that was sampled first (red dots â left side first, blue dots â right side first) in right protected specimen brushes (PSB) from the right lower lobe and left PSBs from the left upper lobe. Rarefied at 1000 sequences. 00019-2017_figureS4
Supplementary Material
M. Aanerud 00019-2017_Aanerud
P. Bakke 00019-2017_Bakke
T. Eagan 00019-2017_Eagan
R. Grønseth 00019-2017_Gronseth
S. Lehmann 00019-2017_Lehmann
