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Heightened response to e-cigarettes in COPD

Jack Bozier, Sandra Rutting, Dia Xenaki, Matthew Peters, Ian Adcock, Brian G. Oliver
ERJ Open Research 2019 5: 00192-2018; DOI: 10.1183/23120541.00192-2018
Jack Bozier
1Respiratory Cellular and Molecular Biology, Woolcock Institute of Medical Research, University of Sydney, Sydney, Australia
2School of Life Sciences, University of Technology Sydney, Sydney, Australia
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Sandra Rutting
1Respiratory Cellular and Molecular Biology, Woolcock Institute of Medical Research, University of Sydney, Sydney, Australia
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Dia Xenaki
1Respiratory Cellular and Molecular Biology, Woolcock Institute of Medical Research, University of Sydney, Sydney, Australia
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Matthew Peters
3Concord Hospital, University of Sydney, Sydney, Australia
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Ian Adcock
4Airway Diseases Section, National Heart and Lung Institute, Imperial College London, London, UK
5Biomedical Research Unit; Section of Respiratory Diseases, Royal Brompton and Harefield NHS Trust, London, UK
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Brian G. Oliver
1Respiratory Cellular and Molecular Biology, Woolcock Institute of Medical Research, University of Sydney, Sydney, Australia
2School of Life Sciences, University of Technology Sydney, Sydney, Australia
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  • For correspondence: brian.oliver@uts.edu.au
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    FIGURE 1

    Primary human airway smooth muscle cells were stimulated with different concentrations of a, e) 18 mg·mL−1 nicotine tobacco-flavoured e-cigarette vapour extract (EVE), b, f) 0 mg·mL−1 nicotine tobacco-flavoured EVE, c, g) 18 mg·mL−1 nicotine menthol-flavoured EVE and d, h) 0 mg·mL−1 nicotine menthol-flavoured EVE for 24 h. The control well contained 0.1% FBS DMEM. a–d) CXCL8 release production was measured in supernatant using ELISA. Error bars represent ±sem. Two-way ANOVA with Tukey's post-test was used for statistical analysis, significance is represented as *: p≤0.05; **: p≤0.01. e–h) Cytotoxicity was determined post-stimulation using an MTT assay. Data are normalised to unstimulated cells and error bars represent ±sem. Two-way ANOVA with Tukey's post-test was used for statistical analysis, significance is represented as *: p≤0.05; ***: p≤0.001. COPD: chronic obstructive pulmonary disease; MTT: 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

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Heightened response to e-cigarettes in COPD
Jack Bozier, Sandra Rutting, Dia Xenaki, Matthew Peters, Ian Adcock, Brian G. Oliver
ERJ Open Research Feb 2019, 5 (1) 00192-2018; DOI: 10.1183/23120541.00192-2018

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Heightened response to e-cigarettes in COPD
Jack Bozier, Sandra Rutting, Dia Xenaki, Matthew Peters, Ian Adcock, Brian G. Oliver
ERJ Open Research Feb 2019, 5 (1) 00192-2018; DOI: 10.1183/23120541.00192-2018
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