Skip to main content

Main menu

  • Home
  • Current issue
  • Early View
  • Archive
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • COVID-19 submission information
    • Institutional open access agreements
    • Peer reviewer login
  • Alerts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • Early View
  • Archive
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • COVID-19 submission information
    • Institutional open access agreements
    • Peer reviewer login
  • Alerts
  • Subscriptions

Because age matters – A comparative multi-omics analysis of young and old mice in the Bleomycin-induced lung fibrosis model

Stephan Klee, Daniel Veyel, Karsten Quast, Kathrin Wenger, Bartlomiej Krawczyk, Wolfgang Rist, Andreas Luippold, Matthew James Thomas, Sorif Uddin, Marc Kästle
ERJ Open Research 2019 5: PP113; DOI: 10.1183/23120541.lungscienceconference-2019.PP113
Stephan Klee
1Boehringer Ingelheim, Department of Immunology and Respiratory Disease Research, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel Veyel
2Boehringer Ingelheim, Department of Drug Discovery Sciences, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karsten Quast
3Boehringer Ingelheim, Target Discovery Research, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kathrin Wenger
2Boehringer Ingelheim, Department of Drug Discovery Sciences, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bartlomiej Krawczyk
2Boehringer Ingelheim, Department of Drug Discovery Sciences, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wolfgang Rist
2Boehringer Ingelheim, Department of Drug Discovery Sciences, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andreas Luippold
2Boehringer Ingelheim, Department of Drug Discovery Sciences, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matthew James Thomas
1Boehringer Ingelheim, Department of Immunology and Respiratory Disease Research, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sorif Uddin
1Boehringer Ingelheim, Department of Immunology and Respiratory Disease Research, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marc Kästle
1Boehringer Ingelheim, Department of Immunology and Respiratory Disease Research, Biberach, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

The Bleomycin (Bleo)-induced lung fibrosis is the most commonly used model to study lung fibrosis in mice. This model resembles a number of key pathological aspects in the fibrotic phase that are found in human IPF patients. However, mice used in experiments are often 3 months of age, which is comparable to a 20-year-old human. On the other hand, patients suffering from IPF are usually older than 60 years. With increasing age, a number of changes occur that are not found in young adolescents, such as impairment of the innate and adaptive immune system (immuno-aging), stem cell exhaustion and changes in the extracellular matrix.

We here compared both young (3 months) and old mice (21 months; equivalent of 60-65 years in humans) in the model of Bleo-induced lung fibrosis. We used a multi-omics approach (transcriptomics, proteomics, lipidomics and metabolomics) to analyse lung tissue and plasma. Our results show that young and old mice are highly similar in regard to fibrotic changes when analyzing the mice 21 days after Bleo instillation, as assessed by lung function measurements, µCT and expression of fibrosis-associated markers in the lung. However, we also identified significant differences between both age groups during the fibrotic phase. We found an upregulation of T- and B-cell markers in old Bleo-treated mice compared to young mice. Furthermore, old mice showed a downregulation of proteins required for proper protein folding and ribosomal proteins, indicating potential unfolded protein response and mitochondrial stress. Comparison with human data sets will help to answer if we can model human lung fibrosis more closely using the Bleo model in aged mice.

Footnotes

Cite this article as: ERJ Open Research 2019; 5 : Suppl. 2, PP113.

This is an ERS Lung Science Conference abstract. No full-text version is available. Further material to accompany this conference is available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2019
Back to top
Vol 5 Issue suppl 2 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Because age matters – A comparative multi-omics analysis of young and old mice in the Bleomycin-induced lung fibrosis model
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Because age matters – A comparative multi-omics analysis of young and old mice in the Bleomycin-induced lung fibrosis model
Stephan Klee, Daniel Veyel, Karsten Quast, Kathrin Wenger, Bartlomiej Krawczyk, Wolfgang Rist, Andreas Luippold, Matthew James Thomas, Sorif Uddin, Marc Kästle
ERJ Open Research Mar 2019, 5 (suppl 2) PP113; DOI: 10.1183/23120541.lungscienceconference-2019.PP113

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Because age matters – A comparative multi-omics analysis of young and old mice in the Bleomycin-induced lung fibrosis model
Stephan Klee, Daniel Veyel, Karsten Quast, Kathrin Wenger, Bartlomiej Krawczyk, Wolfgang Rist, Andreas Luippold, Matthew James Thomas, Sorif Uddin, Marc Kästle
ERJ Open Research Mar 2019, 5 (suppl 2) PP113; DOI: 10.1183/23120541.lungscienceconference-2019.PP113
Reddit logo Technorati logo Twitter logo Connotea logo Facebook logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Partial apoptosis of epithelial cells in Idiopathic Pulmonary Fibrosis(IPF).
  • The RNA binding proteins ZFP36L1 and ZFP36L2 exert genome-wide effects on airways epithelium and glucocorticoid responses
  • Lineage tracing of cells expressing the mesenchymal profibrotic transcription factor Prrx1 in the normal and fibrotic lung
Show more Molecular pathology and functional genomics

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About ERJ Open Research

  • Editorial board
  • Journal information
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Online ISSN: 2312-0541

Copyright © 2023 by the European Respiratory Society