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Ivacaftor decreases monocyte sensitivity to interferon-γ in people with cystic fibrosis

Katherine B. Hisert, Timothy P. Birkland, Kelly Q. Schoenfelt, Matthew E. Long, Brenda Grogan, Suzanne Carter, W. Conrad Liles, Edward F. McKone, Lev Becker, Anne M. Manicone
ERJ Open Research 2020 6: 00318-2019; DOI: 10.1183/23120541.00318-2019
Katherine B. Hisert
1Dept of Medicine, University of Washington, Seattle, WA, USA
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  • For correspondence: hisertk@njhealth.org
Timothy P. Birkland
1Dept of Medicine, University of Washington, Seattle, WA, USA
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Kelly Q. Schoenfelt
2Ben May Dept for Cancer Research, University of Chicago, Chicago, IL, USA
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Matthew E. Long
1Dept of Medicine, University of Washington, Seattle, WA, USA
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Brenda Grogan
3Dept of Medicine, St Vincent's University Hospital, Dublin, Ireland
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Suzanne Carter
3Dept of Medicine, St Vincent's University Hospital, Dublin, Ireland
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W. Conrad Liles
1Dept of Medicine, University of Washington, Seattle, WA, USA
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Edward F. McKone
3Dept of Medicine, St Vincent's University Hospital, Dublin, Ireland
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Lev Becker
2Ben May Dept for Cancer Research, University of Chicago, Chicago, IL, USA
4These authors contributed equally
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Anne M. Manicone
1Dept of Medicine, University of Washington, Seattle, WA, USA
4These authors contributed equally
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Abstract

This study demonstrates that initiation of the CFTR modulator ivacaftor in people with cystic fibrosis and susceptible CFTR mutations causes an acute reduction in blood monocyte sensitivity to the key proinflammatory cytokine IFN-γ http://bit.ly/2TeI6LG

To the Editor:

Management of cystic fibrosis has been revolutionised by the introduction of cystic fibrosis transmembrane conductance regulator (CFTR) modulators. These compounds treat the underlying molecular basis of the disease by increasing activity of defective CFTR channels, which improves many clinical parameters and enhances patient quality of life [1]. Next-generation modulators, also known as triple combination therapy, promise to be highly efficacious in up to 90% of patients [2] and will likely dramatically change the landscape of cystic fibrosis disease. Studies examining individuals before and after initiation of CFTR modulators have revealed novel functions of CFTR and shown that CFTR modulators do not reverse all disease manifestations [3–5]. Thus, knowledge of the post-modulator cystic fibrosis disease state is crucial for understanding what continued therapies will be needed for people with cystic fibrosis and what new challenges may arise.

In prior work, we sought to identify immune pathways that were differentially modulated in the presence and absence of CFTR activity [6]. We isolated monocytes from subjects with G551D-CFTR mutations before and after initiation of the modulator ivacaftor. Using an unbiased proteomics approach, we identified post-ivacaftor changes in the monocyte plasma membrane proteome consistent with decreased interferon (IFN)-γ-related responses. Although both monocytes and macrophages from individuals with cystic fibrosis have been shown to manifest abnormal immune responses, myeloid IFN-γ responses had not previously been reported as altered in people with cystic fibrosis [7]. In the current study, we took advantage of a second cohort of subjects with cystic fibrosis who were initiating ivacaftor to test the hypothesis that ivacaftor dampens monocyte sensitivity to IFN-γ.

We enrolled 10 patients, heterozygous for R117H-CFTR (5T) [8], from the Adult Cystic Fibrosis Clinic at St Vincent's University Hospital, Dublin, Ireland; second CFTR mutations were ΔF508 (n=7), M156R (n=2) and 2662+1G→A (n=1). The median age of subjects was 40.5 years (range 25–52 years), six were male, median body mass index was 25.1 kg·m−2 (range 18.5–32.5 kg·m−2), median baseline sweat chloride was 79 mM (range 61–98 mM) and median baseline forced expiratory volume in 1 s (FEV1) was 72% predicted (range 35–109% predicted). Recent clinical sputum cultures were positive for Pseudomonas aeruginosa in two subjects and Staphylococcus aureus (with or without P. aeruginosa, Haemophilus or Achromobacter species) in five subjects. Three subjects had prior P. aeruginosa-positive cultures and one subject did not produce sputum. Patients were excluded if they had received ivacaftor within 6 months or if they had received antibiotics within 2 weeks of the day 0 visit. Patients were allowed to continue other standard cystic fibrosis therapies. Ivacaftor was initiated following the day 0 visit. No subject had cystic fibrosis-related diabetes. Seven of the subjects were pancreatic sufficient; three subjects were being evaluated for pancreatic insufficiency but had recent normal faecal elastase values. The Research Ethics Committee at St Vincent's University Hospital approved this study and all subjects provided written informed consent.

All subjects experienced a pronounced decrease in sweat chloride between days 0 and 2 of ivacaftor (median decrease 25.5 mM, range 21–44 mM; p<0.0001), which persisted at day 7 (p<0.0001 compared to day 0, data not shown). The cohort also experienced a statistically significant increase in FEV1 at day 2 (median 0.15 L, range 0.02–0.37 L; p=0.0022) and day 7 (median 0.19 L, range 0.05–0.69 L; p=0.0021; data not shown). These findings confirmed that ivacaftor augmented CFTR activity in a clinically relevant manner within 48 h, as seen previously in the G551D-CFTR cohort [5].

We purified monocytes from subjects' blood at days 0, 2 and 7 of treatment, stimulated the cells in vitro with recombinant human IFN-γ (100 ng·mL−1) and quantified IFN-γ target gene expression. Monocytes isolated on day 0 mounted robust IFN-γ-induced transcription of IFN-γ-stimulated genes (ISGs); in contrast, monocytes isolated from the same subjects 7 days after starting ivacaftor had markedly decreased ISG expression in response to IFN-γ stimulation (figure 1a). Monocytes obtained from a subset of subjects (n=6) at day 2 indicated that induction of ISGs was attenuated as early as 48 h post-ivacaftor (figure 1b). The transcriptional responses of pro-inflammatory genes not induced by IFN-γ, such as those encoding tumour necrosis factor and interleukin-6, were not changed following ivacaftor (data not shown).

FIGURE 1
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FIGURE 1

Decreased interferon (IFN)-γ-mediated responses in monocytes isolated from individuals with cystic fibrosis following initiation of ivacaftor therapy. a) Comparison of gene expression 3 h after stimulation ex vivo with IFN-γ (100 ng·mL−1) in monocytes isolated at day D0 and D7 of ivacaftor treatment; HPRT was used as a housekeeping gene. n=10, p-values were calculated using Wilcoxon signed rank testing. Peripheral blood mononuclear cells were recovered from blood by density centrifugation and monocytes were isolated by CD14-positive selection magnetic bead separation (Miltenyi, Auburn, CA, USA). Monocytes were plated with or without recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) in multiwell plates. Cells were lysed with RLT buffer (Qiagen, Valencia, CA, USA) for RNA analysis after 3 h. RNA was isolated using the RNeasy Plus Kit (Qiagen) per manufacturer's instructions. Real-time reverse transcription PCR was performed on total RNA using TaqMan Gene Expression Assays primer/probe sets for target genes. b) Ivacaftor dampens monocyte responses to IFN-γ within 2 days of starting therapy; n=6. *: p<0.05; **: p≤0.005 by ANOVA. c–f) Monocytes isolated from subjects at specified time points after initiation of ivacaftor were exposed to IFN-γ for 1 h then lysed with Laemmli buffer (Alfa Aesar, Haverhill, MA, USA). Immunoblots were performed to determine abundance of STAT1 and phosphorylated STAT1 (pSTAT1); β-actin was used as a housekeeping control protein. c) Representative immunoblots from two subjects showing results from D0 and D7 lysates. d) Densitometry was performed to quantitate changes in pSTAT abundance; n=8, p-value calculated using Wilcoxon signed rank testing. e) Representative immunoblots from subject 3 showing results from D0, D2 and D7. f) Densitometry comparing abundance of pSTAT at day 2 versus day 0; n=4, p-value calculated using Student's t-test. g) Ivacaftor did not cause changes in plasma IFN-γ concentrations during the first week of treatment, as measured by Meso Scale Discovery biomarker assay. Statistical comparisons noted above were performed using Excel (Student's t-test) or GraphPad Prism 7 (Wilcoxon signed rank testing and ANOVA); p<0.05 was considered significant.

IFN-γ signalling is mediated by the JAK–STAT pathway [9]: the IFN-γ receptor activates JAK proteins resulting in phosphorylation of STAT1, which translocates to the nucleus and increases transcription of ISGs. STAT1 protein levels were similar in monocytes recovered before and after initiation of ivacaftor (figure 1c). However, consistent with the decrease in ISG expression, IFN-γ-induced STAT1 phosphorylation was reduced in monocytes recovered on day 7 compared to monocytes isolated before initiation of ivacaftor (figure 1c and d). Decreased phosphorylation of STAT1 in response to IFN-γ exposure was detected within 48 h of subjects beginning ivacaftor (figure 1e and f). Because monocytes can become either primed by or tolerant to IFN-γ based on prior exposure to differential doses of IFN-γ [10], we also evaluated plasma IFN-γ levels. Plasma IFN-γ concentrations were unchanged during the first week of ivacaftor (figure 1g), consistent with analyses from our previous cohort [6].

These data reveal that ivacaftor causes a reduction in acute responsiveness of peripheral blood monocytes to IFN-γ in people with cystic fibrosis, a change that may be clinically relevant. However, because our experiments used a single (likely supraphysiological) dose of IFN-γ, the clinical relevance of our findings remains unclear. Another limitation of this study is that we tested only one timepoint following cell stimulation with IFN-γ, and thus the data do not differentiate between a decreased amplitude of response to IFN-γ or a delayed response. A third limitation is that this study does not demonstrate the mechanism by which ivacaftor decreases monocyte IFN-γ sensitivity. Ivacaftor may be acting by a CFTR-dependent mechanism, as has been described for other cystic fibrosis-related myeloid cell defects [11, 12]. Ivacaftor could also act directly on monocytes by a CFTR-independent mechanism; recent studies observed that CFTR modulators may have immune-dampening effects on macrophages that lack modulator-susceptible CFTR mutations [12, 13]. Finally, reduced monocyte IFN-γ responses could be a secondary effect of ivacaftor. Prior work suggests that cystic fibrosis monocytes are impaired secondary to changes in plasma mediators caused by chronic airway infection [14]; thus, ivacaftor-induced changes in airway bacterial burden and inflammatory cytokines [5] could alter plasma immune factors. In this current cohort, as in our prior cohort [6], we did not detect changes in numbers of peripheral blood mononuclear cells, monocytes, T-cells or B-cells 1 week after initiation of ivacaftor (data not shown); however, ivacaftor may produce other alterations in systemic inflammation not detected by these assays.

Studying primary cells from subjects with cystic fibrosis provides the most clinically relevant characterisation of changes in immune cells after initiation of CFTR modulators; however, this study design produces results that are often challenging to recreate in vitro. Negative results from in vitro studies (e.g. addition of ivacaftor or CFTR inhibitors to freshly isolated monocytes) suggest a lack of direct effect of ivacaftor or CFTR activity on monocyte IFN-γ responses. Yet, because monocytes have rapid turnover in the blood [15], it remains possible that the influence of ivacaftor or CFTR activity on monocyte responses occurs during haematopoiesis and cannot be reproduced in vitro using mature peripheral blood monocytes. Even without a definitive mechanism for how ivacaftor changes monocyte sensitivity to IFN-γ, these findings have intriguing and potentially clinically significant implications, as IFN-γ plays a central role in modulating immune functions and promoting antimicrobial activities [9]. Changes in monocyte IFN-γ responses may contribute to ivacaftor-induced decreases in airway bacterial burden and inflammation [5]. Exuberant myeloid cell IFN-γ-related responses could contribute to pathologic cystic fibrosis airway inflammation, and a reduction in these responses could shift monocytes and macrophages to less inflammatory phenotypes. Alternately, IFN-γ can arrest monocyte migration [16] and dampening of IFN-γ responses could enhance trafficking of pathogen-fighting monocytes to the cystic fibrosis airway. IFN-γ is also important for upregulation of pathogen receptors, as well as antigen processing and presentation, especially of intracellular bacteria such as mycobacterial species [9]; thus, dampening IFN-γ responses could have unexpected negative consequences, causing individuals with cystic fibrosis to become more susceptible to these pathogens. In fact, prior studies of T-cell responses in cystic fibrosis suggested that the cystic fibrosis airway could be an IFN-γ-deficient environment [17, 18], leading to a clinical trial of nebulised IFN-γ in people with cystic fibrosis [19]. This study was halted early due to an increase in exacerbations in the treatment group, highlighting the fact that IFN-γ responses are complex and their role in cystic fibrosis requires further elucidation. Follow-up studies are needed to clarify the contributions of monocyte responses to cystic fibrosis airway inflammation and how initiation of CFTR modulators will modify immune responses in the cystic fibrosis lung.

Acknowledgements

We thank Pradeep Singh (University of Washington, Seattle, WA, USA) for inviting us to join the research team in Dublin, Ireland; we also thank Michael Welsh (University of Iowa, Iowa City, IA, USA), David Stoltz (University of Iowa), Jan Launspach (University of Iowa), Mavra Nasir (Dartmouth University, Hanover, NH, USA), Anina Ratjen (University of Washington), Robert Gray (University of Edinburgh, Edinburgh, UK) and Gareth Hardisty (University of Edinburgh) for collegial interactions and strategic support during specimen collection and initial specimen processing in Dublin.

Footnotes

  • Author contributions: K.B. Hisert, E.F. McKone and L. Becker conceived the hypothesis and designed the study. K.B. Hisert, T.P. Birkland, K.Q. Schoenfelt, B. Grogan, S. Carter, E.F. McKone, L. Becker and A.M. Manicone acquired and analysed the data. K.B. Hisert, M.E. Long, W.C. Liles, L. Becker and A.M. Manicone prepared the manuscript.

  • Support statement: This work was funded by a CF Research Innovation Award from Vertex Pharmaceuticals, Inc. Funding information for this article has been deposited with the Crossref Funder Registry.

  • Conflict of interest: K.B. Hisert reports a CF Research Innovation Award from Vertex Pharmaceuticals during the conduct of the study.

  • Conflict of interest: T.P. Birkland has nothing to disclose.

  • Conflict of interest: K.Q. Schoenfelt has nothing to disclose.

  • Conflict of interest: M.E. Long has nothing to disclose.

  • Conflict of interest: B. Grogan has nothing to disclose.

  • Conflict of interest: S. Carter reports grants from the Irish Thoracic Society, Gilead and the Cystic Fibrosis Association of Ireland during the conduct of the study.

  • Conflict of interest: W.C. Liles has nothing to disclose.

  • Conflict of interest: E.F. McKone reports a grant from Vertex Pharmaceuticals during the conduct of the study; and advisory boards fees from Vertex Pharmaceuticals and Proteostasis, and speaker fees from Roche and Novartis, outside the submitted work.

  • Conflict of interest: L. Becker has nothing to disclose.

  • Conflict of interest: A.M. Manicone has nothing to disclose.

  • Received December 3, 2019.
  • Accepted February 18, 2020.
  • Copyright ©ERS 2020
http://creativecommons.org/licenses/by-nc/4.0/

This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.

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Ivacaftor decreases monocyte sensitivity to interferon-γ in people with cystic fibrosis
Katherine B. Hisert, Timothy P. Birkland, Kelly Q. Schoenfelt, Matthew E. Long, Brenda Grogan, Suzanne Carter, W. Conrad Liles, Edward F. McKone, Lev Becker, Anne M. Manicone
ERJ Open Research Apr 2020, 6 (2) 00318-2019; DOI: 10.1183/23120541.00318-2019

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Ivacaftor decreases monocyte sensitivity to interferon-γ in people with cystic fibrosis
Katherine B. Hisert, Timothy P. Birkland, Kelly Q. Schoenfelt, Matthew E. Long, Brenda Grogan, Suzanne Carter, W. Conrad Liles, Edward F. McKone, Lev Becker, Anne M. Manicone
ERJ Open Research Apr 2020, 6 (2) 00318-2019; DOI: 10.1183/23120541.00318-2019
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