Effect of continuous positive airway pressure treatment on permeability, inflammation and mucus production of human epithelial cells

Sandra Grau-Bartual, Ahmed M. Al-Jumaily, Paul M. Young, Daniela Traini, Maliheh Ghadiri
ERJ Open Research 2020 6: 00327-2019; DOI: 10.1183/23120541.00327-2019

Figures

  • FIGURE 1
    FIGURE 1

    Sealed airtight custom-made 600 cm3 cell holder, cover not included (not to scale), with one inlet connection to generate positive pressure onto the transwell inserts.

  • FIGURE 2
    FIGURE 2

    Epithelial cell layer permeability at control (ΔP=0 cmH2O) and 5, 10, 15 and 20 cmH2O for Calu-3 and RPMI 2650 cells: a) trans-epithelial electrical resistance (TEER) measurements at 1000 Hz and b) apparent permeability coefficient (Papp). Data are presented as mean±sd, n=3. ****: p<0.0001.

  • FIGURE 3
    FIGURE 3

    Bulk modulus (B) and total epithelial cell thickness (ycell) measured before (control) and after 5 and 20 cmH2O continuous positive airway pressure (CPAP) application for Calu-3 and RPMI 2650 cells. Data are presented as mean±sd, n=3. *: p<0.05; ****: p<0.0001.

  • FIGURE 4
    FIGURE 4

    Airway surface liquid (ASL) thickness reduction between 100% and 70% relative humidity scenarios at control (ΔP=0 cmH2O) and 5, 10, 15 and 20 cmH2O for Calu-3 and RPMI 2650 cells. Data are presented as mean±sd, n=3. *: p<0.05; **: p<0.01; ***: p<0.001.

  • FIGURE 5
    FIGURE 5

    Pro-inflammatory marker concentration on Calu-3 cells at control (ΔP=0 cmH2O) and after ΔP=5, 10, 15 and 20 cmH2O continuous positive airway pressure application for 10 min at humidity level 0 and 7: a) interleukin (IL)-6 and b) IL-8. Data are presented as mean±sd, n=3. *: p<0.05.

  • FIGURE 6
    FIGURE 6

    Mucus secretion rate measured with the ratio of blue (RGBB ratio) as a function of time cells at ΔP=0 cmH2O for Calu-3 and RPMI 2650 cells. Data are presented as mean±sd, n=3. ****: p<0.0001.

  • FIGURE 7
    FIGURE 7

    Microscopic images of mucus stained with Alcian blue. CPAP: continuous positive airway pressure. a) Calu-3 cells stained after 8 h under 0 cmH2O CPAP (control). b) Calu-3 cells stained after 8 h under 10 cmH2O CPAP. c) RPMI 2650 cells stained after 8 h under 0 cmH2O CPAP (control). d) RPMI 2650 cells stained after 8 h under 10 cmH2O CPAP. Scale bar: 500 μm.

  • FIGURE 8
    FIGURE 8

    Mucus production measured with the ratio of blue (RGBB ratio) at control (ΔP=0 cmH2O) and ΔP=5, 10, 15 and 20 cmH2O continuous positive airway pressure for 8 h of Calu-3 and RPMI 2650 cells. Data are presented as mean±sd, n=3. ****: p<0.0001.

Supplementary Materials

  • Supplementary Material

    Please note: supplementary material is not edited by the Editorial Office, and is uploaded as it has been supplied by the author.

    Supplementary material 00327-2019.SUPPLEMENT

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Effect of continuous positive airway pressure treatment on permeability, inflammation and mucus production of human epithelial cells
Sandra Grau-Bartual, Ahmed M. Al-Jumaily, Paul M. Young, Daniela Traini, Maliheh Ghadiri
ERJ Open Research Apr 2020, 6 (2) 00327-2019; DOI: 10.1183/23120541.00327-2019
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