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Increased protease-activated receptor 1 autoantibodies are associated with severe COVID-19

Florian Tran, Danielle M.M. Harris, Alena Scharmacher, Hanna Graßhoff, Kristina Sterner, Susanne Schinke, Nadja Käding, Jens Y. Humrich, Otávio Cabral-Marques, Joana P. Bernardes, Neha Mishra, Thomas Bahmer, Jeanette Franzenburg, Bimba F. Hoyer, Andreas Glück, Martina Guggeis, Alexander Ossysek, Andre Küller, Derk Frank, Christoph Lange, Jan Rupp, Jan Heyckendorf, Karoline I. Gaede, Howard Amital, Philip Rosenstiel, Yehuda Shoenfeld, Gilad Halpert, Avi Z. Rosenberg, Kai Schulze-Forster, Harald Heidecke, Gabriela Riemekasten, Stefan Schreiber
ERJ Open Research 2022 8: 00379-2022; DOI: 10.1183/23120541.00379-2022
Florian Tran
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
2Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
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  • For correspondence: f.tran@ikmb.uni-kiel.de
Danielle M.M. Harris
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
3Institute for Human Nutrition and Food Science, University Medical Center Schleswig-Holstein, Kiel, Germany
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Alena Scharmacher
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
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Hanna Graßhoff
4Department of Rheumatology and Clinical Immunology, University Medical Center Schleswig-Holstein, Lübeck, Germany
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Kristina Sterner
4Department of Rheumatology and Clinical Immunology, University Medical Center Schleswig-Holstein, Lübeck, Germany
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Susanne Schinke
4Department of Rheumatology and Clinical Immunology, University Medical Center Schleswig-Holstein, Lübeck, Germany
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Nadja Käding
5Department of Infectious Diseases and Microbiology, University Medical Center Schleswig-Holstein, Lübeck, Germany
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Jens Y. Humrich
4Department of Rheumatology and Clinical Immunology, University Medical Center Schleswig-Holstein, Lübeck, Germany
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Otávio Cabral-Marques
6Department of Immunology, Instituto de Ciencias Biomedicas, Universidade de São Paulo, São Paulo, SP, Brazil
7Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil
8Network of Immunity in Infection, Malignancy, and Autoimmunity, Universal Scientific Education and Research Network, Sao Paulo, SP, Brazil
9Department of Medicine, Division of Molecular Medicine, University of São Paulo School of Medicine, São Paulo, Brazil
10Laboratory of Medical Investigation 29, University of São Paulo School of Medicine, São Paulo, Brazil
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Joana P. Bernardes
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
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Neha Mishra
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
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Thomas Bahmer
2Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
11LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany
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Jeanette Franzenburg
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
12Institute of Clinical Chemistry, University Medical Center Schleswig-Holstein, Kiel, Germany
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Bimba F. Hoyer
13Section for Rheumatology, Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
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Andreas Glück
2Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
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Martina Guggeis
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
2Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
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Alexander Ossysek
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
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Andre Küller
14Department of Internal Medicine III, University Medical Center Schleswig-Holstein, Kiel, Germany
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Derk Frank
14Department of Internal Medicine III, University Medical Center Schleswig-Holstein, Kiel, Germany
15German Centre for Cardiovascular Research (DZHK), partner site Hamburg, Kiel, Lübeck, Germany
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Christoph Lange
16Research Center Borstel, Borstel, Germany
17German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems, Borstel, Germany
18Respiratory Medicine and International Health, University of Lübeck, Lübeck, Germany
19Baylor College of Medicine and Texas Children's Hospital, Houston, TX, USA
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Jan Rupp
5Department of Infectious Diseases and Microbiology, University Medical Center Schleswig-Holstein, Lübeck, Germany
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Jan Heyckendorf
2Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
16Research Center Borstel, Borstel, Germany
17German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems, Borstel, Germany
20Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, Germany
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Karoline I. Gaede
16Research Center Borstel, Borstel, Germany
17German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems, Borstel, Germany
20Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, Germany
21BioMaterialBank Nord, Borstel, Germany
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Howard Amital
22Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Ramat-Gan, Israel
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Philip Rosenstiel
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
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Yehuda Shoenfeld
22Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Ramat-Gan, Israel
23Ariel University, Ariel, Israel
24Laboratory of the Mosaic of Autoimmunity, Saint Petersburg State University, Saint-Petersburg, Russia
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Gilad Halpert
22Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Ramat-Gan, Israel
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Avi Z. Rosenberg
25Department of Pathology, Johns Hopkins University, Baltimore, MD, USA
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Kai Schulze-Forster
26CellTrend GmbH, Luckenwalde, Germany
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Harald Heidecke
26CellTrend GmbH, Luckenwalde, Germany
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Gabriela Riemekasten
3Institute for Human Nutrition and Food Science, University Medical Center Schleswig-Holstein, Kiel, Germany
27Priority Area Asthma and Allergy, Research Center Borstel, Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, Germany
28These authors contributed equally
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Stefan Schreiber
1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
2Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
28These authors contributed equally
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Article Information

vol. 8 no. 4
DOI 
https://doi.org/10.1183/23120541.00379-2022

Published By 
European Respiratory Society
Online ISSN 
2312-0541
History 
  • Received August 1, 2022
  • Accepted August 11, 2022
  • Published online December 27, 2022.

Article Versions

  • previous version (October 13, 2022 - 06:04).
  • You are viewing the most recent version of this article.
Copyright & Usage 
Copyright ©The authors 2022 This version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. For commercial reproduction rights and permissions contact permissions@ersnet.org

Author Information

  1. Florian Tran1,2⇑,
  2. Danielle M.M. Harris1,3,
  3. Alena Scharmacher1,
  4. Hanna Graßhoff4,
  5. Kristina Sterner4,
  6. Susanne Schinke4,
  7. Nadja Käding5,
  8. Jens Y. Humrich4,
  9. Otávio Cabral-Marques6,7,8,9,10,
  10. Joana P. Bernardes1,
  11. Neha Mishra1,
  12. Thomas Bahmer2,11,
  13. Jeanette Franzenburg1,12,
  14. Bimba F. Hoyer13,
  15. Andreas Glück2,
  16. Martina Guggeis1,2,
  17. Alexander Ossysek1,
  18. Andre Küller14,
  19. Derk Frank14,15,
  20. Christoph Lange16,17,18,19,
  21. Jan Rupp5,
  22. Jan Heyckendorf2,16,17,20,
  23. Karoline I. Gaede16,17,20,21,
  24. Howard Amital22,
  25. Philip Rosenstiel1,
  26. Yehuda Shoenfeld22,23,24,
  27. Gilad Halpert22,
  28. Avi Z. Rosenberg25,
  29. Kai Schulze-Forster26,
  30. Harald Heidecke26,
  31. Gabriela Riemekasten3,27,28 and
  32. Stefan Schreiber1,2,28
  1. 1Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel and University Medical Center Schleswig-Holstein, Kiel, Germany
  2. 2Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
  3. 3Institute for Human Nutrition and Food Science, University Medical Center Schleswig-Holstein, Kiel, Germany
  4. 4Department of Rheumatology and Clinical Immunology, University Medical Center Schleswig-Holstein, Lübeck, Germany
  5. 5Department of Infectious Diseases and Microbiology, University Medical Center Schleswig-Holstein, Lübeck, Germany
  6. 6Department of Immunology, Instituto de Ciencias Biomedicas, Universidade de São Paulo, São Paulo, SP, Brazil
  7. 7Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil
  8. 8Network of Immunity in Infection, Malignancy, and Autoimmunity, Universal Scientific Education and Research Network, Sao Paulo, SP, Brazil
  9. 9Department of Medicine, Division of Molecular Medicine, University of São Paulo School of Medicine, São Paulo, Brazil
  10. 10Laboratory of Medical Investigation 29, University of São Paulo School of Medicine, São Paulo, Brazil
  11. 11LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany
  12. 12Institute of Clinical Chemistry, University Medical Center Schleswig-Holstein, Kiel, Germany
  13. 13Section for Rheumatology, Department of Internal Medicine I, University Medical Center Schleswig-Holstein, Kiel, Germany
  14. 14Department of Internal Medicine III, University Medical Center Schleswig-Holstein, Kiel, Germany
  15. 15German Centre for Cardiovascular Research (DZHK), partner site Hamburg, Kiel, Lübeck, Germany
  16. 16Research Center Borstel, Borstel, Germany
  17. 17German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems, Borstel, Germany
  18. 18Respiratory Medicine and International Health, University of Lübeck, Lübeck, Germany
  19. 19Baylor College of Medicine and Texas Children's Hospital, Houston, TX, USA
  20. 20Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, Germany
  21. 21BioMaterialBank Nord, Borstel, Germany
  22. 22Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Ramat-Gan, Israel
  23. 23Ariel University, Ariel, Israel
  24. 24Laboratory of the Mosaic of Autoimmunity, Saint Petersburg State University, Saint-Petersburg, Russia
  25. 25Department of Pathology, Johns Hopkins University, Baltimore, MD, USA
  26. 26CellTrend GmbH, Luckenwalde, Germany
  27. 27Priority Area Asthma and Allergy, Research Center Borstel, Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL), Borstel, Germany
  28. 28These authors contributed equally
  1. Florian Tran (f.tran{at}ikmb.uni-kiel.de)
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Funding

  • BMBF iTREAT project

  • 3TR (IMI2JU)

  • DFG Research Unit 5042 “miTarget”

  • Cluster of Excellence 2167 “Precision Medicine in Chronic Inflammation”

    RTF-VI
  • State Schleswig-Holstein

    COVID-reponse grant

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Increased protease-activated receptor 1 autoantibodies are associated with severe COVID-19
Florian Tran, Danielle M.M. Harris, Alena Scharmacher, Hanna Graßhoff, Kristina Sterner, Susanne Schinke, Nadja Käding, Jens Y. Humrich, Otávio Cabral-Marques, Joana P. Bernardes, Neha Mishra, Thomas Bahmer, Jeanette Franzenburg, Bimba F. Hoyer, Andreas Glück, Martina Guggeis, Alexander Ossysek, Andre Küller, Derk Frank, Christoph Lange, Jan Rupp, Jan Heyckendorf, Karoline I. Gaede, Howard Amital, Philip Rosenstiel, Yehuda Shoenfeld, Gilad Halpert, Avi Z. Rosenberg, Kai Schulze-Forster, Harald Heidecke, Gabriela Riemekasten, Stefan Schreiber
ERJ Open Research Oct 2022, 8 (4) 00379-2022; DOI: 10.1183/23120541.00379-2022

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Increased protease-activated receptor 1 autoantibodies are associated with severe COVID-19
Florian Tran, Danielle M.M. Harris, Alena Scharmacher, Hanna Graßhoff, Kristina Sterner, Susanne Schinke, Nadja Käding, Jens Y. Humrich, Otávio Cabral-Marques, Joana P. Bernardes, Neha Mishra, Thomas Bahmer, Jeanette Franzenburg, Bimba F. Hoyer, Andreas Glück, Martina Guggeis, Alexander Ossysek, Andre Küller, Derk Frank, Christoph Lange, Jan Rupp, Jan Heyckendorf, Karoline I. Gaede, Howard Amital, Philip Rosenstiel, Yehuda Shoenfeld, Gilad Halpert, Avi Z. Rosenberg, Kai Schulze-Forster, Harald Heidecke, Gabriela Riemekasten, Stefan Schreiber
ERJ Open Research Oct 2022, 8 (4) 00379-2022; DOI: 10.1183/23120541.00379-2022
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