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Phenotype and function of innate lymphoid cells in a murine model of impaired muco-ciliary clearance challenged with allergen

khatuna Lobjanidze, Olga Halle, Claudia Kessemeier, Emily Fuchshuber, Antje Munder, Gesine Hansen, Burkhard Tümmler, Anna-Maria Dittrich
ERJ Open Research 2022 8: 110; DOI: 10.1183/23120541.LSC-2022.110
khatuna Lobjanidze
1Department of paediatric pulmonology, allergology and neonatology, Hannover Medical School (MHH), German Centre for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
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  • For correspondence: lobjanidze.khatuna@mh-hannover.de
Olga Halle
1Department of paediatric pulmonology, allergology and neonatology, Hannover Medical School (MHH), German Centre for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
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Claudia Kessemeier
1Department of paediatric pulmonology, allergology and neonatology, Hannover Medical School (MHH), German Centre for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
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Emily Fuchshuber
1Department of paediatric pulmonology, allergology and neonatology, Hannover Medical School (MHH), German Centre for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
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Antje Munder
1Department of paediatric pulmonology, allergology and neonatology, Hannover Medical School (MHH), German Centre for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
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Gesine Hansen
1Department of paediatric pulmonology, allergology and neonatology, Hannover Medical School (MHH), German Centre for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
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Burkhard Tümmler
1Department of paediatric pulmonology, allergology and neonatology, Hannover Medical School (MHH), German Centre for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
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Anna-Maria Dittrich
1Department of paediatric pulmonology, allergology and neonatology, Hannover Medical School (MHH), German Centre for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
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Abstract

Despite the known role of innate lymphoid cells as promoters of pulmonary inflammation in various lung diseases, the understanding of their involvement in conditions of impaired muco-ciliary clearance and the pathogenesis of cystic fibrosis (CF) remains incomplete. In the study presented here, we analysed ILCs in mice with over-expression of the sodium-channel ßENaC, which display a CF-like pulmonary disease phenotype with reduced muco-ciliary clearance and enhanced airway inflammation. We challenged juvenile and adult ßENaC and wild type (WT) mice intra-tracheally with Aspergillus fumigatus (A.f.) extract and compared frequencies and cytokine-production of ILC subpopulations by flow cytometry.

Our research indicates that the response to A.f. is age- and muco-ciliary clearance dependent. In contrast to juvenile WT mice, juvenile ßENaC mice showed increased percentages of effector cytokines interleukin (IL)-5 and IL-13 together with the type 2 innate lymphoid cells. Allergic responses evaluated by BAL differential count and airway eosinophilia was stronger in both, juvenile and adult ßENaC mice compared to WT mice. These data support the previous studies that impaired allergen clearance correlates with the increased levels of innate type-2 lymphoid cells (ILC2) in juvenile ßENaC mice.

Future experiments in ßENaC/RAG−/− vs. ßENaC mice will interrogate the contribution of ILC2s to the exaggerated type-II inflammation observed in ßENaC mice. Understanding the contribution of ILC2s in this age-dependent process contributes to the dissection of age- and muco-ciliary clearance-dependent inflammatory pathways, which apply to pulmonary diseases beyond CF.

  • Experimental approaches
  • Animal models
  • Cystic fibrosis

Footnotes

Cite this article as ERJ Open Research 2022; 8: Suppl. 8, 110.

This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.

This is an ERS Lung Science Conference abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2022
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Phenotype and function of innate lymphoid cells in a murine model of impaired muco-ciliary clearance challenged with allergen
khatuna Lobjanidze, Olga Halle, Claudia Kessemeier, Emily Fuchshuber, Antje Munder, Gesine Hansen, Burkhard Tümmler, Anna-Maria Dittrich
ERJ Open Research Mar 2022, 8 (suppl 8) 110; DOI: 10.1183/23120541.LSC-2022.110

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Phenotype and function of innate lymphoid cells in a murine model of impaired muco-ciliary clearance challenged with allergen
khatuna Lobjanidze, Olga Halle, Claudia Kessemeier, Emily Fuchshuber, Antje Munder, Gesine Hansen, Burkhard Tümmler, Anna-Maria Dittrich
ERJ Open Research Mar 2022, 8 (suppl 8) 110; DOI: 10.1183/23120541.LSC-2022.110
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