Abstract
Sarcoidosis is а disease that affects multiple organs and may lead to organ failure and death. A recent hypothesis states that the human microbiome dysbiosis may contribute to sarcoidosis development. This line of research was not fully explored. In this pilot study we made an attempt to identify the role of the dormant circulatory blood and lung tissue microbiome in sarcoidosis etiology. Blood and lung granulomatous biopsy samples were collected from three age and gender-matching patients. Samples were lysed and mechanically grinded. The human DNA was hydrolyzed with DNAse I. Residual human and microbial cells were centrifuged. Total DNA was extracted from the pellet and sequenced by Illumina NovaSeq. Cleaned data reads were identified taxonomically by Kraken 2 software. Metagenome shotgun sequencing provides the most complete snapshot of the current state of the microbiome since it detects both culturable and nonculturable microorganisms. For direct visualization of the taxonomic content and abundance, we drew a stacked bar chart, Sankey diagram. Few, but highly abundant microbial species were observed both in the blood and tissues samples such as the species Rothia mucilaginosa, Staphylococcus sp., Streptococcus sp. but also species like Sphingomonas taxi, Pseudomonas tolaasii, Bradyrhizobium sp. with unknown opportunistic potential. These findings (1) suggest the presence of active exchange between blood and lung tissue microbiomes and (2) support the notion of microbiome contribution in sarcoidosis development.
ACKNOWLEDGEMENTS
This work was supported by the National Science Programme VIHREN and the Bulgarian National Science Fund (BNSF) with grant N: KP-06-DB/10 - 21.12.2019
Footnotes
Cite this article as ERJ Open Research 2022; 8: Suppl. 8, 215.
This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.
This is an ERS Lung Science Conference abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).
- Copyright ©the authors 2022