Skip to main content

Main menu

  • Home
  • Current issue
  • Early View
  • Archive
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • COVID-19 submission information
    • Institutional open access agreements
    • Peer reviewer login
  • Alerts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • Early View
  • Archive
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • COVID-19 submission information
    • Institutional open access agreements
    • Peer reviewer login
  • Alerts
  • Subscriptions

Influenza infection of epithelial cells alters extracellular vesicle microRNA involved in viral replication and the immune response

Laura Reid, C. Mirella Spalluto, Karl Staples, Tom Wilkinson
ERJ Open Research 2022 8: 77; DOI: 10.1183/23120541.LSC-2022.77
Laura Reid
1University of Southampton, Southampton, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: lvr2g18@soton.ac.uk
C. Mirella Spalluto
1University of Southampton, Southampton, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karl Staples
1University of Southampton, Southampton, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tom Wilkinson
1University of Southampton, Southampton, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

Influenza A virus (IAV) is a major health threat both in terms of seasonal epidemics and global pandemics. Recent studies have demonstrated epithelial cells release lipid bilayer particles, termed extracellular vesicles (EVs) that are selectively packaged with microRNA (miR) which regulate gene expression. We investigated changes in the miR profile of EVs released from bronchial epithelial cells in response to infection with IAV.

EVs were isolated by size exclusion chromatography from the apical surface wash of air-liquid interface differentiated bronchial epithelial cells (BCi-NS1.1) either uninfected (n=5) or infected (n=5) with IAV (Wisconsin/67/2005). EV miR was then sequenced and reads were mapped to miRBase and the human genome.

Differential expression analyses identified 6 miR that were significantly altered in EVs released from IAV infected BCi compared to uninfected BCi (Bonferroni p<0.05). Of these, 5 were upregulated (fold change >1.5): miR-155-5p, miR-122-5p, miR-378a-3p, miR-7-5p and miR-146a-5p and 1 was downregulated (fold change <−1.5): miR-505-5p. MiR target prediction and functional analysis was performed using miRNet. Influenza A KEGG pathway was identified to be significantly enriched. Furthermore, Gene Ontology revealed relevant biological processes, such as apoptosis, cell death, cell cycle, nuclear transport, NF-κB cascade, cytokine-mediated signalling and viral reproductive process to be regulated by these miRs. Future in vitro modelling will validate these findings.

EV miRs may be a key mechanism in modulating the response to IAV and therefore are potential novel targets for future anti-viral therapies.

  • Viruses
  • Immunology
  • Epithelial cell

Footnotes

Cite this article as ERJ Open Research 2022; 8: Suppl. 8, 77.

This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.

This is an ERS Lung Science Conference abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).

  • Copyright ©the authors 2022
Previous
Back to top
Vol 8 Issue suppl 8 Table of Contents
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Influenza infection of epithelial cells alters extracellular vesicle microRNA involved in viral replication and the immune response
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Influenza infection of epithelial cells alters extracellular vesicle microRNA involved in viral replication and the immune response
Laura Reid, C. Mirella Spalluto, Karl Staples, Tom Wilkinson
ERJ Open Research Mar 2022, 8 (suppl 8) 77; DOI: 10.1183/23120541.LSC-2022.77

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Influenza infection of epithelial cells alters extracellular vesicle microRNA involved in viral replication and the immune response
Laura Reid, C. Mirella Spalluto, Karl Staples, Tom Wilkinson
ERJ Open Research Mar 2022, 8 (suppl 8) 77; DOI: 10.1183/23120541.LSC-2022.77
Reddit logo Technorati logo Twitter logo Connotea logo Facebook logo Mendeley logo

Jump To

  • Article
  • Info & Metrics
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • The metabolite succinate inhibits influenza virus replication through succinylation and nuclear retention of the viral nucleoprotein
  • A human ex vivo model to study the innate immune response to NTHi in the lung
  • Alteration of lung defense mechanisms after exposure to urban coarse PM leads to infection by Streptococcus pneumoniae
Show more Respiratory infections and bronchiectasis

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About ERJ Open Research

  • Editorial board
  • Journal information
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Online ISSN: 2312-0541

Copyright © 2023 by the European Respiratory Society