Skip to main content

Main menu

  • Home
  • Current issue
  • Early View
  • Archive
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • COVID-19 submission information
    • Institutional open access agreements
    • Peer reviewer login
  • Alerts
  • Subscriptions
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

User menu

  • Log in
  • Subscribe
  • Contact Us
  • My Cart

Search

  • Advanced search
  • ERS Publications
    • European Respiratory Journal
    • ERJ Open Research
    • European Respiratory Review
    • Breathe
    • ERS Books
    • ERS publications home

Login

European Respiratory Society

Advanced Search

  • Home
  • Current issue
  • Early View
  • Archive
  • Authors/reviewers
    • Instructions for authors
    • Submit a manuscript
    • COVID-19 submission information
    • Institutional open access agreements
    • Peer reviewer login
  • Alerts
  • Subscriptions

Imprinting of bronchial epithelial cells upon in vivo rhinovirus infection in asthma patients

Abilash Ravi, Saheli Chowdhury, Annemiek Dijkhuis, Barbara S. Dierdorp, Tamara Dekker, Rianne Kruize, Yanaika S. Sabogal Piñeros, Christof J. Majoor, Peter J. Sterk, René Lutter
ERJ Open Research 2021; DOI: 10.1183/23120541.00522-2021
Abilash Ravi
1Department of Respiratory Medicine, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
2Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Abilash Ravi
Saheli Chowdhury
2Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Annemiek Dijkhuis
2Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Barbara S. Dierdorp
2Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tamara Dekker
2Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rianne Kruize
2Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yanaika S. Sabogal Piñeros
1Department of Respiratory Medicine, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
2Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christof J. Majoor
1Department of Respiratory Medicine, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter J. Sterk
1Department of Respiratory Medicine, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
René Lutter
1Department of Respiratory Medicine, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands
2Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam Infection and Immunity Institute, Amsterdam, Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for René Lutter
  • For correspondence: r.lutter@amsterdamumc.nl
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Background Defective translocation of the translational repressor TIAR (T-cell internal antigen receptor) in bronchial epithelial cells (BECs) from asthma patients underlies epithelial hyperresponsiveness, reflected by an exaggerated production of a select panel of inflammatory cytokines like CXCL-8, IL-6, G-CSF, CXCL-10, upon exposure to TNF and IL-17A. With this study we aimed to clarify whether epithelial hyperresponsiveness is a consistent finding, is changed upon in vivo exposure to rhinovirus-A16 (RV-A16) and, also applies to the bronchoconstrictor endothelin-1.

Methods BECs were obtained from asthma patients (n=18) and healthy individuals (n=11), 1 day before and 6 days post RV-A16 exposure. BECs were cultured and stimulated with TNF and IL-17A and inflammatory mediators were analysed. The bronchoalveolar lavage fluid (BALF) was obtained in parallel with BECs to correlate differential cell counts and inflammatory mediators with epithelial hyperresponsiveness.

Results Epithelial hyperresponsiveness was confirmed in sequential samples and even increased in BECs from asthma patients after RV-A16 exposure, but not in BECs from healthy individuals. Endothelin-1 tended to increase in BECs from asthma patients collected after RV-A16 exposure, but not in BECs from healthy individuals. In vitro CXCL-8 and endothelin-1 production correlated. In vivo relevance for in vitro CXCL-8 and endothelin-1 production was shown by correlations with FEV1% predicted and CXCL-8 BALF levels.

Conclusion Epithelial hyperresponsiveness is an intrinsic defect in BECs from asthma patients, which increases upon viral exposure, but not in BECs from healthy individuals. This epithelial hyperresponsiveness also applies to the bronchoconstrictor endothelin-1, which could be involved in airway obstruction.

Footnotes

This manuscript has recently been accepted for publication in the ERJ Open Research. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJOR online. Please open or download the PDF to view this article.

Conflict of interest: Abilash Ravi has nothing to disclose.

Conflict of interest: Saheli Chowdhury has nothing to disclose.

Conflict of interest: Annemiek Dijkhuis has nothing to disclose.

Conflict of interest: Barbara S. Dierdorp has nothing to disclose.

Conflict of interest: Tamara Dekker has nothing to disclose.

Conflict of interest: Rianne Kruize has nothing to disclose.

Conflict of interest: Yanaika S. Sabogal Piñeros has nothing to disclose.

Conflict of interest: Christof J. Majoor has nothing to disclose.

Conflict of interest: Peter J. Sterk has nothing to disclose.

Conflict of interest: Rene Lutter reports support for the present manuscript from the Netherlands Asthma Foundation now Netherlands Lung Fund (grant no: 3-2-07-012; 3.2.10.069) and GlaxoSmithKline (CRT 114696).

This is a PDF-only article. Please click on the PDF link above to read it.

  • Received August 26, 2021.
  • Accepted November 25, 2021.
  • Copyright ©The authors 2021
http://creativecommons.org/licenses/by-nc/4.0/

This version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. For commercial reproduction rights and permissions contact permissions{at}ersnet.org

PreviousNext
Back to top
Vol 8 Issue 3 Table of Contents
ERJ Open Research: 8 (3)
  • Table of Contents
  • Index by author
Email

Thank you for your interest in spreading the word on European Respiratory Society .

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Imprinting of bronchial epithelial cells upon in vivo rhinovirus infection in asthma patients
(Your Name) has sent you a message from European Respiratory Society
(Your Name) thought you would like to see the European Respiratory Society web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
Citation Tools
Imprinting of bronchial epithelial cells upon in vivo rhinovirus infection in asthma patients
Abilash Ravi, Saheli Chowdhury, Annemiek Dijkhuis, Barbara S. Dierdorp, Tamara Dekker, Rianne Kruize, Yanaika S. Sabogal Piñeros, Christof J. Majoor, Peter J. Sterk, René Lutter
ERJ Open Research Jan 2021, 00522-2021; DOI: 10.1183/23120541.00522-2021

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Imprinting of bronchial epithelial cells upon in vivo rhinovirus infection in asthma patients
Abilash Ravi, Saheli Chowdhury, Annemiek Dijkhuis, Barbara S. Dierdorp, Tamara Dekker, Rianne Kruize, Yanaika S. Sabogal Piñeros, Christof J. Majoor, Peter J. Sterk, René Lutter
ERJ Open Research Jan 2021, 00522-2021; DOI: 10.1183/23120541.00522-2021
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Full Text (PDF)

Jump To

  • Article
    • Abstract
  • Info & Metrics
  • PDF

Subjects

  • Respiratory infections and tuberculosis
  • Lung biology and experimental studies
  • Asthma and allergy
  • Tweet Widget
  • Facebook Like
  • Google Plus One

More in this TOC Section

  • Vasoreactive phenotype in children with pulmonary arterial hypertension and syncope
  • BMI increase – A risk factor for FEV1 decline for overweight and obese adults with asthma
  • Phase I studies of BI 1015550, a preferential PDE4B inhibitor, in healthy males and patients with idiopathic pulmonary fibrosis
Show more Original research article

Related Articles

Navigate

  • Home
  • Current issue
  • Archive

About ERJ Open Research

  • Editorial board
  • Journal information
  • Press
  • Permissions and reprints
  • Advertising

The European Respiratory Society

  • Society home
  • myERS
  • Privacy policy
  • Accessibility

ERS publications

  • European Respiratory Journal
  • ERJ Open Research
  • European Respiratory Review
  • Breathe
  • ERS books online
  • ERS Bookshop

Help

  • Feedback

For authors

  • Instructions for authors
  • Publication ethics and malpractice
  • Submit a manuscript

For readers

  • Alerts
  • Subjects
  • RSS

Subscriptions

  • Accessing the ERS publications

Contact us

European Respiratory Society
442 Glossop Road
Sheffield S10 2PX
United Kingdom
Tel: +44 114 2672860
Email: journals@ersnet.org

ISSN

Online ISSN: 2312-0541

Copyright © 2022 by the European Respiratory Society