Airflow limitation and tongue microbiota in community-dwelling elderly individuals
- Toru Takeshita1,2,9,
- Koichiro Matsumoto3,9,
- Michiko Furuta1,
- Satoru Fukuyama3,
- Kenji Takeuchi1,4,
- Hiroaki Ogata3,5,
- Mikari Asakawa1,
- Shinya Kageyama1,
- Jun Hata6,7,
- Toshiharu Ninomiya6,7,
- Hiromasa Inoue8 and
- Yoshihisa Yamashita1⇑
- 1Section of Preventive and Public Health Dentistry, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
- 2OBT Research Center, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
- 3Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
- 4Department of Preventive medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan
- 5Department of Respiratory Medicine, National Hospital Organization Fukuoka National Hospital, Fukuoka, Japan
- 6Department of Epidemiology and Public Health, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
- 7Center for Cohort Studies, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
- 8Department of Pulmonary Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan
- 9These authors contributed equally to this work
- Prof. Yoshihisa Yamashita, Section of Preventive and Public Health Dentistry, Division of Oral Health, Growth, Development, Kyushu University Faculty of Dental Science, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. E-mail: yoshi{at}dent.kyushu-u.ac.jp
Abstract
Background Numerous oral indigenous microorganisms are constantly introduced into the stomach via the laryngopharynx, and a portion of these microorganisms irregularly reaches the lower airways and lungs. This study investigated the association between airflow limitation and the status of tongue microbiota, which is a primary source of ingested oral bacterial populations.
Methods The study population consisted of 484 community-dwelling adults aged 70–80 years inhabiting Hisayama town, who underwent a regular health examination including dental examination and spirometry test in 2016. The bacterial density and composition of their tongue microbiota were determined using the 16S rRNA gene in our previous study to understand their relationship with oral health conditions [1]. The present cross-sectional study compared the tongue microbiota status between elderly individuals with airflow limitation and those with normal airflow.
Results The total bacterial density of the tongue microbiota of individuals with airflow limitation was significantly higher than that of individuals with normal airflow. Logistic regression analysis demonstrated that a high-biomass tongue microbiota was significantly associated with airflow limitation after adjustment for smoking intensity and other covariates (adjusted odds ratio=1.61, 95% confidence interval=1.01–2.60). Of the predominant commensals, higher amounts of Prevotella melaninogenica and Actinomyces odontolyticus were associated with a higher prevalence of airflow limitation.
Conclusion These results indicate that increased bacterial burden in the tongue microbiota is associated with a higher prevalence of airflow limitation.
Footnotes
This manuscript has recently been accepted for publication in the ERJ Open Research. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJOR online. Please open or download the PDF to view this article.
Conflict of interest: Dr. Takeshita has nothing to disclose.
Conflict of interest: Dr. Matsumoto has nothing to disclose.
Conflict of interest: Dr. Furuta has nothing to disclose.
Conflict of interest: Dr. Fukuyama has nothing to disclose.
Conflict of interest: Dr. Takeuchi has nothing to disclose.
Conflict of interest: Dr. Ogata has nothing to disclose.
Conflict of interest: Dr. Asakawa has nothing to disclose.
Conflict of interest: Dr. Kageyama has nothing to disclose.
Conflict of interest: Dr. Hata has nothing to disclose.
Conflict of interest: Dr. Ninomiya has nothing to disclose.
Conflict of interest: Dr. Inoue has nothing to disclose.
Conflict of interest: Dr. Yamashita has nothing to disclose.
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- Received August 27, 2020.
- Accepted January 22, 2021.
- ©The authors 2021
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