TY - JOUR T1 - Lipoxin A<sub>4</sub> promotes lung epithelial repair whilst inhibiting fibroblast proliferation JF - ERJ Open Research JO - erjor DO - 10.1183/23120541.00079-2015 VL - 2 IS - 3 SP - 00079-2015 AU - Shengxing Zheng AU - Vijay K. D'Souza AU - Domokos Bartis AU - Rachel C.A. Dancer AU - Dhruv Parekh AU - Babu Naidu AU - Fang Gao-Smith AU - Qian Wang AU - Shengwei Jin AU - Qingquan Lian AU - David R. Thickett Y1 - 2016/07/01 UR - http://openres.ersjournals.com/content/2/3/00079-2015.abstract N2 - Therapy that promotes epithelial repair whilst protecting against fibroproliferation is critical for restoring lung function in acute and chronic respiratory diseases.Primary human alveolar type II cells were used to model the effects of lipoxin A4 in vitro upon wound repair, proliferation, apoptosis and transdifferention. Effects of lipoxin A4 upon primary human lung fibroblast proliferation, collagen production, and myofibroblast differentiation were also assessed.Lipoxin A4 promoted type II cell wound repair and proliferation, blocked the negative effects of soluble Fas ligand/tumour necrosis factor α upon cell proliferation, viability and apoptosis, and augmented the epithelial cell proliferative response to bronchoaveolar lavage fluid (BALF) from acute respiratory distress syndrome (ARDS). In contrast, Lipoxin A4 reduced fibroblast proliferation, collagen production and myofibroblast differentiation induced by transforming growth factor β and BALF from ARDS. The effects of Lipoxin A4 were phosphatidylinositol 3′-kinase dependent and mediated via the lipoxin A4 receptor.Lipoxin A4 appears to promote alveolar epithelial repair by stimulating epitheial cell wound repair, proliferation, reducing apoptosis and promoting trans-differentiation of alveolar type II cells into type I cells. Lipoxin A4 reduces fibroblast proliferation, collagen production and myofibroblast differentiation. These data suggest that targeting lipoxin actions may be a therapeutic strategy for treating the resolution phase of ARDS.Lipoxin A4 promotes epithelial repair while inhibiting fibroproliferation in vitro in human alveolar epithelial cells http://ow.ly/SxMu301cBRP ER -