TY - JOUR T1 - Effects of IQOS on macrophage viability and function JF - ERJ Open Research JO - erjor DO - 10.1183/23120541.lungscienceconference-2019.PP130 VL - 5 IS - suppl 2 SP - PP130 AU - Shanon MALELA AU - Aaron Scott AU - David Thickett AU - Gurjinder Sandhar Y1 - 2019/03/07 UR - http://openres.ersjournals.com/content/5/suppl_2/PP130.abstract N2 - Heat not burn (HNB) devices are being sold as a less harmful alternative to traditional cigarettes. The newest HNB device from Philip Morris, IQOS, produces a smoke free tobacco vapour through which nicotine can be delivered. IQOS heats the glycerine impregnated tobacco stick to form a vapour instead of smoke. The device literature implies the sub-combustion temperature reduces of levels of harmful components. There is an urgent need to investigate potential adverse effects.Objective: To investigate the effects of IQOS vapour extract (IVE) and cigarette smoke extract (CSE) on THP-1 macrophage viability and function.Methods: Macrophages were differentiated from THP-1 monocytes by PMA challenge for 24 hours then rested for 3 days. CSE and IVE were produced by bubbling 3 Kentucky research cigarettes / 3 tobacco sticks through 10 ml media. Viability was assessed by Cell titre aqueous assay. Apoptosis and necrosis were assessed by flow cytometry using Annexin V / PI staining. Phagocytosis was assessed by Phrodo assay.Results: Both CSE and IVE produced a dose dependent reduction in viability after 24 hours, with significant loss of viability after 3% CSE and IVE challenge. Apoptosis was significantly increased after 6 hours with 3% and 5% CSE/IVE. There was a trend towards increased necrosis however this was not significant currently. There was no significant difference in apoptosis between CSE and IVE. Phagocytosis was significantly inhibited by CSE and IVE at 2% however CSE showed greater inhibition than IVE at all concentrations.Conclusion: Exposure to CSE and IVE produced similar toxic and inhibitory effects on THP-1 macrophages. As such these preliminary studies highlight potential toxicity concerns regarding use of IQOS devices.FootnotesCite this article as: ERJ Open Research 2019; 5 : Suppl. 2, PP130.This is an ERS Lung Science Conference abstract. No full-text version is available. Further material to accompany this conference is available at www.ers-education.org (ERS member access only). ER -