RT Journal Article
SR Electronic
T1 Expanding clinical phage microbiology: simulating phage inhalation for respiratory tract infections
JF ERJ Open Research
JO erjor
FD European Respiratory Society
SP 00367-2021
DO 10.1183/23120541.00367-2021
A1 Shira Ben Porat
A1 Daniel Gelman
A1 Ortal Yerushalmy
A1 Sivan Alkalay-Oren
A1 Shunit Coppenhagen-Glazer
A1 Malena Cohen-Cymberknoh
A1 Eitan Kerem
A1 Israel Amirav
A1 Ran Nir-Paz
A1 Ronen Hazan
YR 2021
UL http://openres.ersjournals.com/content/early/2021/08/12/23120541.00367-2021.abstract
AB Phage therapy is a promising antibacterial strategy for resistant respiratory tract infections. Phage inhalation may serve this goal; however, it requires a careful assessment of their delivery by this approach. Here we present an in-vitro model to evaluate phage inhalation. Eight phages, most of which target cystic fibrosis (CF)-common pathogens, were aerosolized by jet nebulizer and administered to a real-scale computed tomography (CT)-derived 3D airways model with a breathing simulator. Viable phage loads reaching the output of the nebulizer and the tracheal level of the model were determined and compared to the loaded amount. Phage inhalation resulted in a diverse range of titer reduction, primarily associated with the nebulization process. No correlation was found between phage delivery to the phage physical or genomic dimensions. These findings highlight the need for tailored simulations of phage delivery, ideally by a patient-specific model in addition to proper phage matching, to increase the potential of phage therapy success.FootnotesThis manuscript has recently been accepted for publication in the ERJ Open Research. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJOR online. Please open or download the PDF to view this article.Conflict of interests: The authors of "Simulating Phage Inhalation for Respiratory Tract Infections" by Ben Porat and Gelman et al. declare no conflict of interest.