RT Journal Article
SR Electronic
T1 Expanding clinical phage microbiology: simulating phage inhalation for respiratory tract infections
JF ERJ Open Research
JO erjor
FD European Respiratory Society
SP 00367-2021
DO 10.1183/23120541.00367-2021
VO 7
IS 4
A1 Shira Ben Porat
A1 Daniel Gelman
A1 Ortal Yerushalmy
A1 Sivan Alkalay-Oren
A1 Shunit Coppenhagen-Glazer
A1 Malena Cohen-Cymberknoh
A1 Eitan Kerem
A1 Israel Amirav
A1 Ran Nir-Paz
A1 Ronen Hazan
YR 2021
UL http://openres.ersjournals.com/content/7/4/00367-2021.abstract
AB Phage therapy is a promising antibacterial strategy for resistant respiratory tract infections. Phage inhalation may serve this goal; however, it requires a careful assessment of their delivery by this approach. Here we present an in vitro model to evaluate phage inhalation. Eight phages, most of which target pathogens common in cystic fibrosis, were aerosolised by jet nebuliser and administered to a real-scale computed tomography-derived 3D airways model with a breathing simulator. Viable phage loads reaching the output of the nebuliser and the tracheal level of the model were determined and compared to the loaded amount. Phage inhalation resulted in a diverse range of titre reduction, primarily associated with the nebulisation process. No correlation was found between phage delivery to the phage physical or genomic dimensions. These findings highlight the need for tailored simulations of phage delivery, ideally by a patient-specific model in addition to proper phage matching, to increase the potential of phage therapy success.Phage therapy can be used against infectious diseases if personally tailored. Using a 3D airway model, this study shows that phage delivery to the respiratory tract by inhalation is unpredictable and requires precise evaluation. https://bit.ly/3ATdzr5