PT  - JOURNAL ARTICLE
AU  - Sangeetha Ramu
AU  - Christian Woehlk
AU  - Juan-Jose Nieto-Fontarigo
AU  - Samuel Cerpes
AU  - Sara Vázquez-Mera
AU  - Mandy Menzel
AU  - Hamid Akbharshahi
AU  - Celeste Porsbjerg
AU  - Lena Uller
TI  - Comparing epithelial alarmin responses to different aeroallergens in bronchial epithelial cells from allergic asthma patients
AID  - 10.1183/23120541.LSC-2022.83
DP  - 2022 Mar 10
TA  - ERJ Open Research
PG  - 83
VI  - 8
IP  - suppl 8
4099  - http://openres.ersjournals.com/content/8/suppl_8/83.short
4100  - http://openres.ersjournals.com/content/8/suppl_8/83.full
SO  - erjor2022 Mar 10; 8
AB  - Background: Exposure of the airway epithelium to allergens is a major trigger of allergic asthma inflammation potentially involving release of epithelial alarmins; TSLP, IL33 and IL25 and metabolite danger associated molecular patterns, ATP and uric acid (UA). In turn these alarmins orchestrate type2 inflammatory responses in asthma.Aim: To compare the alarmin response to four different aeroallergens in human bronchial epithelial cells (HBECs) from patients with allergic asthma.Methods: Primary HBECs from allergic asthmatic donors (n=8) were cultured in vitro and exposed to House dust mite (HDM; 20µg/mL), Alternaria alternata (200µg/mL), Betula pendula (200µg/mL) and Artemisia Vulgaris (1000µg/mL) for 3 and 24h. Gene expression of TSLP and IL33 was analysed by RT-qPCR, whereas TSLP, IL33 and IL25 protein levels were measured by ELISA. Extracellular release of UA was measured by biochemical assay.Results: All four allergens induced TSLP and IL33 gene expression at 3h compared to unstimulated cells. However, only HDM induced a significant release of IL33 and TSLP an early time-point at 3h with TSLP being sustained at 24h. Further, only HDM induced UA release in HBECs compared to unstimulated cells. There was a trend to IL25 release in response to A. alternata.Conclusion: The four different aeroallergens differed as regards effects on alarmin expression and release in HBECs obtained from individuals with allergic asthma. All allergens induced early gene expression of TSLP and IL33, but HDM stood out by also causing release of TSLP (3h and 24h), IL33 (3h), and UA (24h). These data support the view that HDM is a major pathogenic allergen in allergic asthma.FootnotesCite this article as ERJ Open Research 2022; 8: Suppl. 8, 83.This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.This is an ERS Lung Science Conference abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).