RT Journal Article
SR Electronic
T1 Eosinophil-associated gene signature expression in U-BIOPRED severe asthma
JF ERJ Open Research
JO erjor
FD European Respiratory Society
SP 156
DO 10.1183/23120541.LSC-2022.156
VO 8
IS suppl 8
A1 Janice Koranteng
A1 Nazanin Zounemat Kermani
A1 Yusef Badi
A1 Ian Adcock
A1 Charalambos Michaeloudes
A1 Kian Fan Chung
A1 Pankaj Bhavsar
YR 2022
UL http://openres.ersjournals.com/content/8/suppl_8/156.abstract
AB Background: Severe eosinophilic asthma is a phenotype of asthma associated with increased eosinophils in blood and sputum.Objective: Do eosinophil and anti-Interleukin (IL)-5/IL-5 receptor biologic gene signatures define different aspects of the severe asthma phenotypes?Methods: We used a gene signature from blood eosinophil upregulated transcripts in asthmatics and two anti-IL-5 antibody (benralizumab and mepolizumab)-derived gene signatures from blood and bronchoalveolar lavage cells, respectively, and used gene set variation analysis (GSVA) to calculate enrichment scores of these signatures in the blood, sputum and bronchial brushings transcripts of severe asthma.Results: The signatures were enriched in severe Type 2 (T2)-high asthma in bronchial brushings (Pavlidis, S. et al. Eur Respir J 2019; 53: 1800938) (Fig 1A-C). Enrichment of transcriptomic associated cluster (TAC)1 eosinophilic phenotype for all 3 signatures and of neutrophilic TAC2 (Kuo, C-HS. et al. Eur Respir J 2017 49: 1602135) for the mepolizumab signature is shown (Fig 1D-F). Conclusions: Use of these different signatures of eosinophil functionalities provides a more accurate phenotype of the eosinophilic severe asthma than an eosinophil blood count. This would explain the variable expression of these eosinophil signatures in the pauci-granulocytic TAC3.FootnotesCite this article as ERJ Open Research 2022; 8: Suppl. 8, 156.This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.This is an ERS Lung Science Conference abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).