RT Journal Article
SR Electronic
T1 Profiling systemic inflammation and neutrophil function in hospitalized patients with COVID19: results from PREDICT-COVID19
JF ERJ Open Research
JO erjor
FD European Respiratory Society
SP 130
DO 10.1183/23120541.LSC-2022.130
VO 8
IS suppl 8
A1 Merete Long
A1 Holly R Keir
A1 Yan Hui Giam
A1 Andrew J M Howden
A1 Alejandro J Brenes
A1 Christina Rollings
A1 Thomas Pembridge
A1 Lilia Delgado
A1 Hani Abo-Leyah
A1 Rebecca C Hull
A1 Amy Gilmour
A1 Chloe Hughes
A1 Rebecca Dowey
A1 Helena Turton
A1 Benjamin J M New
A1 David W Connell
A1 Hollian Richardson
A1 Roger Thompson
A1 Alison M Condliffe
A1 Angus I Lamond
A1 Diane M Cassidy
A1 Amelia Shoemark
A1 Doreen A Cantrell
A1 James D Chalmers
YR 2022
UL http://openres.ersjournals.com/content/8/suppl_8/130.abstract
AB Introduction: COVID19 can cause profound systemic inflammation. Understanding inflammation and immune cell function may allow prediction of outcomes, longer-term effects and identification of further therapeutic targets.Methods: Prospective observational study of patients with PCR-confirmed SARS-CoV-2 enrolled within 96 hours of admission at two UK hospitals(May2020–March2021), with longitudinal sampling up to 29 days. mRNAseq was performed in peripheral blood cells, 45 serum cytokines were measured with Olink Target48 panel, isolated neutrophils were processed for proteomic and functional analyses. Severe disease was defined as requirement for ventilatory support or death within 28 days of admission.Results: 176 COVID19 patients were included (mean age 64.9years, SD13.6), 101 were male (57.4%). 56 patients developed severe disease (31.8%), mortality was 16.5%. A subset underwent neutrophil proteomics (n=81). 19 serum cytokines were significantly associated with severity (p<0.05; ROC analysis), CCL7 had the strongest discriminatory value (AUC 0.78, 95%CI 0.70-0.85). There were 317 significant differentially expressed genes (Wald test with Benjanini-Hochberg correction; p<0.05) at baseline comparing those discharged or still hospitalised at 4 weeks; pathways included complement and B cell activation. Neutrophil proteomics showed upregulation of interferon signalling, which decreased over time but was not linked to severity. Cytokine and toll like receptor levels, plus the eosinophil-associated protein PRG2, were associated with disease severity and outcome.Conclusion: Proteomic and transcriptomic approaches identified changes associated with patient outcomes.FootnotesCite this article as ERJ Open Research 2022; 8: Suppl. 8, 130.This article was presented at the 2022 ERS Lung Science Conference, in session “Poster Session 2”.This is an ERS Lung Science Conference abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only).