Concentrations of chemokines and cytokines in bronchoalveolar lavage supernatant of the patients and controls based on multiplex antibody bead assay
| Patients | Controls | p-value# | IMA | LPA | p-value¶ | |
| Subjects n | 70 | 8 | 38 | 25 | ||
| Chemokines pg·mL−1 | ||||||
| GRO-α/CXCL1 | 793.70±74.10 | 749.79±210.38 | >0.05 | |||
| IP-10/CXCL10 | 7974.18±1213.38 | 504.28±149.80 | 0.001 | 9714.73±1693.40 | 3716±872.05 | 0.001 |
| MCP-1/CCL2 | 332.81±81.04 | 26.04±11.36 | 0.016 | 369.17±139.31 | 231.71±66.89 | 0.431 |
| MIP-1a/CCL3 | 3.25±1.14 | 2.61±0.81 | >0.05 | |||
| MIP-1b/CCL4 | 68.40±14.98 | 10.99±3.58 | 0.007 | 58.04±12.83 | 42.70±12.99 | 0.214 |
| RANTES/CCL5 | 93.20±17.11 | 21.22±12.04 | 0.037 | 97.87±23.87 | 84.48±26.90 | 0.844 |
| EOTAXIN/CCL11 | 36.69±2.80 | 31.83±1.57 | >0.05 | |||
| Growth factors pg·mL−1 | ||||||
| bFGF | – | – | ||||
| HGF | 705.37±171.45 | 67.62±21.90 | 0.002 | 666.34±185.62 | 642.70±368.56 | 0.577 |
| VEGF | 5071.85±1097.98 | 366.74±98.96 | 0.025 | 5824.14±1778.28 | 3550±1280.30 | 0.684 |
| Interleukins pg·mL−1 | ||||||
| IL-1b | 5.62±2.19 | 3.71±1.16 | >0.05 | |||
| IL-1ra | 196.64±98.19 | 89.82±54.40 | >0.05 | |||
| IL-2 | – | – | ||||
| IL-4 | 3.86±1.35 | 3.41±2.92 | >0.05 | |||
| IL-5 | 2.41±0.78 | 1.18±0.64 | >0.05 | |||
| IL-6 | 59.44±14.46 | 2.5±1.07 | 0.007 | 50.99±19.29 | 47.66±17.59 | 0.800 |
| IL-7 | 42.38±12.26 | 27.65±18.27 | >0.05 | |||
| IL-8 | 224.86±51.04 | 29.17±6.82 | 0.023 | 167.32±30.52 | 154.41±49.36 | 0.140 |
| IL-9 | 36.00±2.87 | 27.42±11.12 | >0.05 | |||
| IL-10 | 6.12±1.04 | 5.80±3.99 | >0.05 | |||
| IL-12 (p70) | 35.06±5.33 | 17.65±10.56 | >0.05 | |||
| IL-13 | 1.70±0.55 | 2.63±1.18 | >0.05 | |||
| IL-15 | 1.31±0.19 | 0.76±0.24 | >0.05 | |||
| IL-17 | 34.33±4.43 | 27.60±18.23 | >0.05 | |||
| Other cytokines pg·mL−1 | ||||||
| G-CSF | 57.23±10.47 | 11.18±6.56 | 0.016 | 39.79±6.44 | 71.16±22.84 | 0.474 |
| GM-CSF | 9.05±1.47 | 15.30±5.87 | >0.05 | |||
| IFNγ | 59.82±14.18 | 18.22±9.99 | 0.041 | 133.64±33.58 | 58.77±16.67 | 0.100 |
| TNFα | – | – | ||||
| PDGFβ | 81.66±18.40 | 27.76±14.53 | >0.05 | |||
| Cell adhesion molecule pg·mL−1 | ||||||
| VCAM-1 | 1173.66±236 | 292.50±189.04 | >0.05 |
Data are presented as mean±SEM, unless otherwise stated. The illustrated results are from one experiment and representative of results from three experiments. All values are the means of triplicate samples. – designates undetectable levels. IMA: invasive mucinous adenocarcinoma; LPA: lepidic predominant adenocarcinoma. #: comparison of concentrations using the Mann–Whitney test; ¶: comparison of concentrations using the Mann–Whitney test. For each occurrence, concentrations in IMA, LPA and control were all significantly different, according to Kruskal–Wallis test.