Antibodies used for immunohistochemistry
| Antibody | Species | Dilution | Clone | Origin | Secondary antibody |
| Tryptase | Mouse | 1:12 000 | G3 | Chemicon, (Temecula, CA, USA) | EnVision G|2 Doublestain System directly labelled with Alexa Fluor 488 |
| Chymase | Mouse | 1:100 | CC1 | Novocastra (Newcastle upon Tyne, UK) | EnVision G|2 Doublestain System (Dako, Glostrup, Denmark) |
| CD34 | Mouse | 1:300 | QBEnd/10 | Dako, (Glostrup, Denmark) | Invitrogen (Eugene, OR, USA) |
| VCAM-1 | Mouse | 1:100 | 1.4C3 | Invitrogen Molecular Probes (Eugene, OR, USA) | EnVision Detection system, Dako |
| Ki-67 | Rabbit | 1:100 | Biocare (Pacheco, CA, USA) | Invitrogen (Eugene, OR, USA) | |
| ITGA4 | Rabbit | 1:50 | LifeSpan Biosciences (Seattle, WA, USA) | Invitrogen (Eugene, OR, USA) | |
| ITGB1 | Rabbit | 1:50 | LifeSpan Biosciences (Seattle, WA, USA) | Invitrogen (Eugene, OR, USA) |
Heat-induced antigen retrieval was performed in PT link (Dako, Glostrup, Denmark) with EnVision FLEX Target retrieval solution high pH (Dako). VCAM: vascular cell adhesion molecule; ITGA4: integrin α4; ITGB1: integrin β1.