Phenotypic and genotypic antibiotic susceptibility results
| Isolate | M. abscessus subsp. | Date of isolate | Isolate type | Clarithromycin MIC at 14 days µg·mL−1 | Constitutive clarithromycin resistance by 23S rRNA gene sequencing | Functional erm(41) by sequencing | Amikacin MIC µg·mL−1 | Amikacin resistance by 16S rRNA gene sequencing |
| A1 | abscessus | Jan 24 2006 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| A2 | abscessus | March 22 2016 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| B1 | abscessus | Oct 12 2005 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| B2 | abscessus | June 10 2016 | Rough | >16 (R) | No | Yes | 32 (I) | No |
| C1 | abscessus | Feb 6 2006 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| C2-1 | abscessus | April 8 2015 | Rough | >16 (R) | No | Yes | 32 (I) | No |
| C2-2 | abscessus | April 8 2015 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| D1-1 | abscessus | Nov 8 2005 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| D1-2 | abscessus | Nov 8 2005 | Smooth | >16 (R) | No | Yes | 32 (I) | No |
| D2 | abscessus | July 13 2016 | Rough | >16 (R) | No | Yes | 32 (I) | No |
| E1# | abscessus | Aug 26 2008 | Smooth | >16 (R) | No | Yes | 8 (S) | No |
| E2-1# | massiliense | Sept 1 2016 | Rough | 1 (S) | No | No | 8 (S) | No |
| E2-2# | massiliense | Sept 1 2016 | Smooth | 0.12 (S) | No | No | 4 (S) | No |
| F1 | abscessus | Nov 29 2007 | Rough | >16 (R) | No | Yes | 8 (S) | No |
| F2 | abscessus | Aug 4 2016 | Rough | >16 (R) | No | Yes | 8 (S) | No |
| G1 | abscessus | July 10 2007 | Rough | 16 (R) | No | Yes | 32 (I) | No |
| G2 | abscessus | Aug 12 2015 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| H1 | abscessus | July 16 2008 | Smooth | >16 (R) | No | Yes | 16 (S) | No |
| H2-1 | abscessus | Dec 1 2015 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| H2-2 | abscessus | Dec 1 2015 | Smooth | 16 (R) | No | Yes | 16 (S) | No |
| I1 | massiliense | Aug 5 2008 | Rough | >16 (R) | No | Yes | 8 (S) | No |
| I2 | massiliense | Nov 4 2015 | Rough | >16 (R) | No | Yes | 8 (S) | No |
| J1 | abscessus | May 11 2010 | Smooth | >16 (R) | No | Yes | 8 (S) | No |
| J2 | abscessus | Sept 21 2016 | Smooth | 8 (R) | No | Yes | 32 (I) | No |
| K1 | abscessus | Sept 21 2010 | Rough | >16 (R) | No | Yes | 16 (S) | No |
| K2 | abscessus | June 12 2016 | Rough | >16 (R) | Yes | Yes | >64 (R) | Yes |
| L1 | abscessus | Jan 4 2011 | Smooth | >16 (R) | No | Yes | 32 (I) | No |
| L2 | abscessus | June 2 2016 | Smooth | >16 (R) | No | Yes | 16 (S) | No |
| M1 | massiliense | Nov 10 2009 | Rough | >16 (R) | Yes | No | 16 (S) | No |
| M2 | massiliense | Nov 10 2015 | Rough | >16 (R) | Yes | No | 8 (S) | No |
| N1¶ | abscessus | April 2 2009 | Smooth | >16 (R) | No | Yes | 4 (S) | No |
| N2-1¶ | massiliense | April 20 2016 | Rough | <0.06 (S) | No | No | 16 (S) | No |
| N2-2¶ | massiliense | April 20 2016 | Smooth | 1 (S) | No | No | 16 (S) | No |
| O1 | abscessus | June 9 2010 | Smooth | >16 (R) | No | Yes | 16 (S) | No |
| O2-1 | abscessus | June 9 2016 | Smooth | >16 (R) | No | Yes | 16 (S) | No |
| O2-2 | abscessus | June 9 2016 | Rough | >16 (R) | No | Yes | 8 (S) | No |
| P1 | massiliense | June 26 2007 | Rough | >16 (R) | Yes | No | 32 (I) | No |
| P2 | massiliense | Oct 19 2011 | Rough | >16 (R) | Yes | No | 16 (S) | No |
MIC: minimum inhibitory concentration; rRNA: ribosomal RNA; R: resistant; S: susceptible; I: intermediate. #: for the E isolates, based on sequencing of four genes, E1 was a different strain from isolates E2-1 and E2-2, which were genetically indistinguishable from each other; ¶: for the N isolates, all three were genetically distinct from each other and were classified as different strains.