Demographic data of cases in the validation study
| Characteristics | aPAP | sPAP | hPAP | Other lung diseases |
| Subjects n | 213 | 40 | 5 | 162 |
| Male/female n | 122/60 | 19/21 | 0/5 | 86/75 |
| Age years mean±sd | 52.6±14.7 | 57.6±13.3 | 56.4±14.5 | 66.2±12.0 |
| Asian/Caucasian n | 213/0 | 40/0 | 5/0 | 162/0 |
| Serum GMAb concentration U·mL−1 | ||||
| Mean±sd | 103.6±127.5 | 0.191±0.302 | 0.148±0.141 | 0.517±.818 |
| Maximum | 899.9 | 1.9 | 0.38 | 48.57 |
| Minimum | 5.59 | 0.04 | 0.02 | 0.01 |
| Diagnostic procedures n | ||||
| BAL | 213 | 36 | 5 | 144 |
| VATS | 0 | 4 | 0 | 17 |
| Autopsy | 0 | 0 | 0 | 1 |
| Other lung diseases n | ||||
| CTD | 45 | |||
| DIPD | 20 | |||
| IIPs | 60 | |||
| IPF | 8 | |||
| Other IIPs | 52 | |||
| Infectious disease | 19 | |||
| Miscellaneous | 18 |
Connective tissue disease (CTD) cases included five amyopathic dermatomyositis cases, 11 polymyositis/dermatomyositis interstitial lung diseases, 11 rheumatoid arthritis cases, four Sjögren syndromes, five systemic scleroses and nine other diseases. Infectious disease cases included eight Pneumocystis pneumonias, four bacterial pneumonias, three influenza pneumonias and four other infectious diseases. Miscellaneous diseases included four alveolar haemorrhages, three chronic eosinophilic pneumonias, two acute respiratory distress syndromes, two chronic hypersensitivity pneumonias and seven other diseases. aPAP: autoimmune pulmonary alveolar proteinosis; sPAP: secondary pulmonary alveolar proteinosis; hPAP: hereditary pulmonary alveolar proteinosis; GMAb: granulocyte–macrophage colony-stimulating factor autoantibody; BAL: bronchoalveolar lavage; VATS: video-assisted thoracic surgery; DIPD: drug-induced pulmonary disease; IIP: idiopathic interstitial pneumonia; IPF: idiopathic pulmonary fibrosis.