Comparison of RNA concentration and gene expression across methods
| Manually selected plugs | Whole sample sputum | Intraclass correlation | |
| RNA concentration ng·µL−1 | 5.10 (3.5–6.9) | 47.6 (25.1–132.0) | 0.93 |
| CLC expression | 0.09 (0.01–0.33) | 0.15 (0.07–0.54) | 0.84 |
| CPA3 expression | 0.07 (0.02–0.33) | 0.20 (0.00–0.60) | 0.81 |
| DNASE1L3 expression | 0.26 (0.11–0.50) | 0.39 (0.23–5.3) | 0.91 |
| IL1B expression | 52.6 (32.2–107.0) | 114 (49.5–246.4) | 0.77 |
| ALPL expression | 1.8 (0.62–4.83) | 4.84 (1.80–9.34) | 0.74 |
| CXCR2 expression | 5.63 (3.66–24.3) | 12.32 (6.93–19.0) | 0.83 |
Data are presented as median (interquartile range) unless otherwise stated. Gene expression presented as cycle threshold change (2−ΔCT) multiplied by 10 000.