Sample | Patient# | Sex | Age years | Associated localised lung lesions | VP1 | ST | LT | Conclusion |
1 | PLCH SS | F | 59 | NA | − | − | − | Negative |
2 | PLCH SS | F | 60 | NA | − | − | − | Negative |
3 | PLCH SS | M | 30 | NA | − | − | − | Negative |
4 | PLCH MS | M | 35 | NA | + | − | − | Positive |
5 | PLCH SS | F | 34 | NA | − | − | − | Negative |
6 | PLCH SS | F | 53 | NA | + | + | + | Positive |
7 | PLCH SS | F | 61 | NA | − | − | − | Negative |
8 | PLCH SS | M | 52 | NA | + | + | − | Positive |
9 | PLCH SS | M | 40 | NA | − | − | − | Negative |
10 | Smoker | F | 72 | Adenocarcinoma | − | + | − | Positive |
11 | Smoker | M | 80 | Adenocarcinoma | − | + | + | Positive |
12 | Smoker | F | 73 | Squamous cell carcinoma | + | − | − | Positive |
13 | Smoker | F | 60 | Adenocarcinoma | − | − | − | Negative |
14 | Smoker | M | 66 | Adenocarcinoma | − | − | + | Positive |
15 | Smoker | F | 70 | Adenocarcinoma | + | + | − | Positive |
16 | Smoker | M | 71 | Secondary adenocarcinoma | − | + | − | Positive |
17 | Smoker | M | 59 | Adenocarcinoma | + | − | − | Positive |
18 | Smoker | M | 75 | Adenocarcinoma | − | − | + | Positive |
19 | Nonsmoker | F | 33 | Localised benign cavity | − | − | + | Positive |
20 | Nonsmoker | F | 28 | Localised cystic lesion | − | − | − | Negative |
21 | Nonsmoker | F | 83 | Adenocarcinoma | + | − | + | Positive |
22 | Nonsmoker | F | 59 | Carcinoid tumour | − | + | − | Positive |
23 | Nonsmoker | F | 60 | Secondary adenocarcinoma | + | + | + | Positive |
24 | Nonsmoker | F | 71 | Squamous cell carcinoma | − | − | − | Negative |
25 | Nonsmoker | F | 86 | Adenocarcinoma | + | − | − | Positive |
26 | Nonsmoker | F | 54 | Localised bronchiectasis | − | − | − | Negative |
27 | Nonsmoker | F | 74 | Secondary adenocarcinoma | − | + | − | Positive |
28 | Nonsmoker | F | 55 | Carcinoid tumour | + | + | + | Positive |
MCC skin biopsies | NA | +++ + ++ | ++ +++ +++ | +++ +++ ++ | Positive |
Detection by real-time PCR of genes coding for VP1, small T (ST) and large T (LT) is reported with a qualitative score as follows. +: cycle threshold (Ct) values between <40 and ≥35; ++: Ct values between <35 and ≥30; +++: Ct values <30. Results were considered negative (−) if there was no detection or a Ct value ≥40. MCC: Merkel cell carcinoma; SS: single-system disease; MS: multisystem disease. #: all PLCH patients were current smokers at the time of surgical lung biopsy; NA: not applicable.