Examined bacterial species collected from nasal lavage, sputum, saliva and toothbrush of adults free from Pseudomonas aeruginosa (AN) lung colonisation at different visits during the study
| Patient ID | Sample | Visit 1 | Visit 2 | Visit 3 | Visit 4 | Visit 5 | No. samples |
| AN1 | Nasal lavage | n | n | n | n | – | 4 |
| Sputum | n | n | n | n | – | 4 | |
| Saliva | n | n | n | – | – | 3 | |
| Toothbrush | n | n | AXY | – | – | 3 | |
| AN2 | Nasal lavage | AXY | PAE | n | – | – | 3 |
| Sputum | AXY | AXY, SAU | AXY | – | – | 3 | |
| Saliva | AXY | PAE, SAU | – | – | – | 2 | |
| Toothbrush | n | – | – | – | – | 1 | |
| AN3 | Nasal lavage | SAU | n | SAU | – | – | 3 |
| Sputum | SAU | SAU, SMA | SAU | – | – | 3 | |
| Saliva | SAU, SMA | SAU | – | – | – | 2 | |
| Toothbrush | n | n | – | – | – | 2 | |
| AN4 | Nasal lavage | AXY | n | – | – | – | 2 |
| Sputum | AXY | SAU, SMA | – | – | – | 2 | |
| Saliva | – | – | – | – | – | 0 | |
| Toothbrush | – | – | – | – | – | 0 | |
| AN5 | Nasal lavage | n | n | n | – | – | 3 |
| Sputum | AXY | AXY | AXY | – | – | 3 | |
| Saliva | AXY, SEM | AXY, SMA | – | – | – | 2 | |
| Toothbrush | n | SEM | – | – | – | 2 | |
| AN6 | Nasal lavage | n | n | n | – | – | 3 |
| Sputum | SEM | n | n | – | – | 3 | |
| Saliva | n | n | – | – | – | 2 | |
| Toothbrush | n | n | – | – | – | 2 | |
| AN7 | Nasal lavage | n | n | SAU | – | – | 3 |
| Sputum | SAU, SMA | SAU | SAU | – | – | 3 | |
| Saliva | SAU | SAU | – | – | – | 2 | |
| Toothbrush | n | n | – | – | – | 2 | |
| AN8 | Nasal lavage | n | SAU | SAU | – | – | 3 |
| Sputum | SMA | SAU | SAU, SMA | – | – | 3 | |
| Saliva | SMA, SAU | n | – | – | – | 2 | |
| Toothbrush | n | – | – | – | – | 1 | |
| AN9 | Nasal lavage | PAE | PAE, SAU | PAE, SAU | PAE, SAU | PAE, SAU | 5 |
| Sputum | PAE, AXY | PAE, SAU, AXY | AXY | AXY | AXY, SAU | 5 | |
| Saliva | SAU, AXY | – | – | – | – | 1 | |
| Toothbrush | n | n | – | – | – | 2 | |
| AN10 | Nasal lavage | n | n | AXY | – | – | 3 |
| Sputum | AXY | AXY | AXY | – | – | 3 | |
| Saliva | SMA | AXY, SMA | – | – | – | 2 | |
| Toothbrush | n | n | – | – | – | 2 | |
| AN11 | Nasal lavage | n | n | n | – | – | 3 |
| Sputum | SAU | SAU | n | – | – | 3 | |
| Saliva | SAU | – | – | – | – | 1 | |
| Toothbrush | SEM | – | – | – | – | 1 | |
| AN12 | Nasal lavage | SAU | SAU | SAU | – | – | 3 |
| Sputum | n | SAU | SAU | – | – | 3 | |
| Saliva | SAU | SAU | SAU | – | – | 3 | |
| Toothbrush | n | SAU | SAU | – | – | 3 | |
| AN13 | Nasal lavage | SAU | SAU | SAU | – | – | 3 |
| Sputum | SAU | SAU | SAU | – | – | 3 | |
| Saliva | – | – | – | – | – | 0 | |
| Toothbrush | – | – | – | – | – | 0 | |
| AN14 | Nasal lavage | SMA | n | SMA | n | – | 4 |
| Sputum | SMA | SMA | SMA | SMA | – | 4 | |
| Saliva | SMA | SMA | – | – | – | 2 | |
| Toothbrush | n | n | – | – | – | 2 | |
| AN15 | Nasal lavage | SAU | SAU | – | – | – | 2 |
| Sputum | SAU | SAU | – | – | – | 2 | |
| Saliva | SAU | – | – | – | – | 1 | |
| Toothbrush | – | – | SAU | n | – | 2 |
PAE: Pseudomonas aeruginosa; SAU: Staphylococcus aureus; SMA: Stenotrophomonas maltophilia; AXY: Achromobacter xylosoxidans; SEM: Serratia marcescens; n: no isolation of the investigated species; –: sample not available. Number of samples for each patient is also indicated. Patients with no saliva samples nor toothbrushes were not included in the collection of these samples.