TABLE 2

DNA methylation at cytosine-phosphate-guanine dinucleotide sites (CpGs) at earlier ages that showed consistent direction of associations with lung function at later age between the Isle of Wight birth cohort (IOWBC) and the Avon Longitudinal Study of Parents and Children (ALSPAC)

Lung function measureCpGsChromosome numberGeneLocation#IOWBCALSPAC
CoefficientpRAWpFDRCoefficientp-value
FEV1
cg1072955714ANKRD9Intergenic−0.180.00080.035−2.020.22
cg165828032WNT10AIntergenic−0.160.00020.0280.590.034
cg1731533111HINFPTSS2000.060.00030.0281.600.84
cg2617445419EFNA2Intergenic0.230.00070.0350.440.15
cg275991297ZNF727Intergenic−0.230.0010.035−0.060.86
FEV1/FVC
cg187608356NRN1Intergenic−0.010.00040.041−0.220.33
cg212408617DNAJB6TSS200−0.0040.00140.041−0.050.95
cg2760119816C16orf87Intergenic0.010.00110.0410.080.28

Coefficients of IOWBC-ALSPAC consistent CpGs for the association of DNA methylation at earlier age with lung function at a later age. The CpGs that showed the same direction of associations and were significant at 0.05 level are shown in bold font. FEV1: forced expiratory volume in 1 s; FVC: forced vital capacity; pFDR: p-value after multiple testing correction by controlling false discovery rate; pRAW: p-value without multiple testing adjustment. #: genes located at intergenic locations were not found in the Illumina annotation file and were identified using the online tool SNIPPER.