TABLE 5

Differentially methylated regions (DMRs; containing ≥2 cytosine-phosphate-guanine dinucleotide sites (CpGs)) of lung function at later age in relation to DNA methylation at earlier age identified by the DMRcate method

Molecular location of DMR (chromosome: start – end)CpGs nStoufferAnnotated gene
Male
 FVC
chr4: 2819770-282047940SH3BP2
chr8: 11659832-1166073330FDFT1, RP11
chr3: 194014481-19401474531.30×10−198CPN2
 FEV1
chr4: 1004525-100467830FGFRL1
chr6: 33084825-3308503130HLA-DPB2#
chr15: 30163660-3016382532.00×10−203TJP1#
chr6: 2891973-289215039.07×10−66SERPINB9#
 FEV1/FVC
chr6: 30038929-30039435100RNF39#
chr19: 1467008-146703230APC2#
chr6: 33871907-3387286134.70×10−188MIR1275#
Female
 FVC
chr22: 30476089-3047652557.80×10−280HORMAD2, CTA
chr6: 88757302-8875739257.46×10−74SPACA1
chr1: 92352293-9235248130TGFBR3
chr10: 135191624-13519223030PAOX, AL360181.1-201
chr11: 2322500-232280830TSPAN32, C11orf21
chr20: 25677290-2567758230ZNF337
chr5: 112824497-11282476537.90×10−254MCC
chr17: 56744332-5674449039.80×10−216RNU1
chr10: 34408530-3440865433.50×10−139PARD3#
chr15: 99975310-9997547031.30×10−107LRRC28#
 FEV1
chr5: 1867978-186869352.75×10−84IRX4#
chr17: 45949743-4594987840SP6#
chr6: 32016257-3201722940TNXB
chr22: 30476089-3047652546.50×10−182HORMAD2, CTA
chr11: 18433554-1843374545.93×10−39LDHC
chr6: 110720918-11072134948.70×10−26DDO#
chr7: 157512397-15751370730PTPRN2#
chr7: 150037890-15003889833.60×10−219RARRES2
chr6: 32294470-3229457731.30×10−190HNRNPA1P2
chr20: 62328084-6232842737.10×10−181TNFRSF6B
chr10: 34408530-3440865432.30×10−139PARD3#
chr15: 99975310-9997547034.30×10−108LRRC28#
chr8: 37605517-3760578336.66×10−95RP11-109A6.3
chr17: 154420-15467131.37×10−88RPH3AL
 FEV1/FVC
chr1: 2058230-205908630PRKCZ#
chr17: 40936570-4093736230WNK4
chr20: 44829602-4482982130CDH22#
chr2: 113993052-11399403533.10×10−156PAX8-AS1
chr7: 57471759-5747236731.80×10−131MIR3147
chr1: 43814764-4381503532.22×10−21MPL

DMRcate annotates to UCSC RefGene from the Illumina annotation file. FEV1: forced expiratory volume in 1 s; FVC: forced vital capacity. #: genes were not found in the Illumina annotation file and were identified using the online tool SNIPPER.