TABLE 4

Association of DNA methylation (DNA-M) with gene expression in the Isle of Wight birth cohort (IOWBC)

CpGsChromosome numberGene nameLocationDNA-M at age 18years with gene expression at 26yearsDNA-M at age 26years with gene expression at 26years
Coefficientp-valueCoefficientp-value
Model 1: CpGs identified for main effects
 FEV1
cg1072955714ANKRD9Intergenic0.100.73−0.0530.77
cg1731533111HINFPTSS2000.0430.10
 FEV1/FVC
cg187608356NRN1Intergenic−0.0720.21
cg212408617DNAJB6TSS200−0.010.62
cg2760119816C16orf87Intergenic0.0210.77
Model 2: CpGs identified for interactions effects
 FVC
cg0137607911SSH3TSS1500−0.170.011−0.170.0033
cg0723038010SCDTSS1500−0.0040.88
cg075576901TGFBR3TSS15001.072.0×10−40.102.7×10−7
cg1408360320ZGPATBody−0.0580.690.180.20
cg2302642011PPP2R1BTSS2000.0010.990.0810.36
 FEV1
cg159818512AGAP1Body−0.630.028−0.230.29
cg165828032WNT10AIntergenic−0.200.31
cg197362862MSH6TSS200−0.120.58−0.380.0165
 FEV1/FVC
cg090599881HORMAD11st Exon; 5′UTR−0.160.620.180.52
cg2003816919GMIP1st Exon; 5′UTR0.0210.38
cg233704665TRIM41Intergenic0.0110.84−0.0280.52

Coefficients of IOWBC-ALSPAC (Avon Longitudinal Study of Parents and Children) consistent cytosinephosphate-guanine dinucleotide sites (CpGs) that had available gene expression data for the association of DNA-M with gene expression both longitudinally and cross-sectionally. CpGs that showed significant association at 0.05 level are shown in bold font. FEV1: forced expiratory volume in 1 s; FVC: forced vital capacity; 5′UTR: 5′ untranslated region.