TABLE 3

DNA methylation at cytosine-phosphate-guanine dinucleotide sites (CpGs) at earlier age that showed consistent sex-specific association with lung function at later age between the Isle of Wight birth cohort (IOWBC) and the Avon Longitudinal Study of Parents and Children (ALSPAC)

Lung function measureCpGsSexChromosome numberGene nameLocation#IOWBCALSPAC
CoefficientpRAWpFDRCoefficientp-value
FVC
cg0137607911SSH3TSS15000.090.380.220.49
cg01376079×sexMale−0.410.00190.042−0.710.12
cg0723038010SCDTSS1500−0.020.25−4.770.62
cg07230380×sexMale0.050.00120.04216.210.23
cg075576901TGFBR3TSS15000.300.00140.210.56
cg07557690×sexMale−0.410.00170.042−0.400.46
cg1408360320ZGPATBody−0.49<0.0001−0.550.76
cg14083603×sexMale0.690.00070.0426.220.018
cg2302642011PPP2R1BTSS2000.170.00592.960.66
cg23026420×sexMale−0.290.00190.041−11.860.19
FEV1
cg159818512AGAP1Body0.070.270.100.77
cg15981851×sexMale−0.360.00020.015−0.0570.91
cg165828032WNT10AIntergenic−0.090.77−0.480.21
cg16582803×sexMale−0.290.00090.032−0.230.67
cg197362862MSH6TSS2000.090.410.230.90
cg19736286×sexMale−0.560.00110.032−1.360.59
FEV1/FVC
cg023979346H2BC13Intergenic−0.010.035−0.0940.46
cg02397934×sexMale0.030.00040.0240.210.25
cg086501258LY6HIntergenic0.040.00140.010.73
cg08650125×sexMale−0.060.00050.0236−0.0290.50
cg090599881HORMAD11st Exon; 5′UTR0.010.36−0.0120.89
cg09059988×sexMale−0.060.00080.025−0.030.79
cg2003816919GMIP1st Exon; 5′UTR−0.02<0.0001−0.590.66
cg20038169×sexMale0.020.00070.0251.8230.34
cg233704665TRIM41Intergenic−0.020.0045−0.0320.43
cg23370466×sexMale0.040.00020.0240.0400.49

Coefficients of IOWBC-ALSPAC consistent CpGs for the sex-specific association of DNA methylation at earlier age with lung function at a later age. The CpGs showed the same direction of associations and were significant at 0.05 level are shown in bold font. FEV1: forced expiratory volume in 1 s; FVC: forced vital capacity; pFDR: p-value after multiple testing correction by controlling false discovery rate; pRAW: p-value without multiple testing adjustment; 5′UTR: 5′ untranslated region. #: genes located at intergenic locations were not found in the Illumina annotation file and were identified using the online tool SNIPPER.