Adaptations in forskolin-induced swelling (FIS) protocol of original 96-well format to allow a 384-well format
| 96-well format, as described by Vonk et al. [11] | 384-well format | |
| 1 | Plates are prewarmed at 37°C prior to organoid-matrigel addition | Plates are precooled at −20°C, and kept on ice during organoid-matrigel plating |
| 2 | 4 μL 50% matrigel organoid suspension is added per well as drops | 10 μL 25% matrigel organoid suspension is added per well with an automatic multichannel, to cover the whole surface |
| 3 | – | Plate is spun down in a centrifuge to ensure matrigel coverage of the whole well surface |
| 4 | After 10 min at 37°C, 50 μL medium per well is added | After 10 min at 37°C, 8 μL medium per well is added |
| 5 | X/Y/Z location is manually set for each well | X/Y/Z is automatically set based on plate lay-out and autofocus of 12 reference points |