Original ArticleSputum Induction in Routine Clinical Care of Children with Cystic Fibrosis
Section snippets
Methods
This is a cross-sectional comparative study in children attending the outpatient CF clinic at the Hospital for Sick Children in Toronto. Inclusion criteria were diagnosis of CF (by sweat chloride ≥60 mmol/L and/or two confirmed disease-causing CFTR mutations), age between 6 and 18 years, ability to perform pulmonary function tests, and forced expiratory volume in 1 second (FEV1) ≥30% predicted.15 Exclusion criteria were acute respiratory distress or hypoxia (oxygen saturation <92% in room air),
Results
Of 120 patients approached for the study between December 2006 and May 2008, 95 (79%) agreed to participate (Table I). There was no significant difference in the baseline characteristics between the study population and the entire CF population meeting the inclusion criteria at the center (n = 200) (data not shown). The duration of the whole sputum induction procedure ranged from 30 to 85 minutes (median, 49 minutes). The median duration of each cycle of nebulization of HS was 7 minutes (range,
Discussion
We assessed the utility of sputum induction in patients with CF in routine clinical setting. This is also the first study to use a high-output electronic nebulizer (PARI eFlow) for the nebulization of hypertonic saline solutions for sputum induction. Sputum induction, with increasing concentrations of hypertonic saline was safe and tolerated by the majority of the patients in our study. There was a discrepancy in the culture results between IS and conventional airway samples in 27% of patients,
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Supported by a grant from the Lynne and Arnold Irwin Foundation. The authors declare no conflicts of interest.
Registered at ClinicalTrials.gov: NCT00721071