Abstract
Probiotics can be considered nonspecific adjuvants of innate immune response by modulating the Th1/Th2 response in different pathological conditions. Our goal is to investigate the probiotic Lactobacillus bulgaricus (Lb) anti-inflammatory effect in experimental asthma. BALB/c-mice were sensitized with ovalbumin (OVA) on days 0 and 7 and then were challenged from day 14-28 (3-times/week) with aerosolized OVA. From seventh day before the sensitization until day 28, mice were treated 3-times/ week with Lb via gavage. All measurements were performed 24 hours after the last OVA challenge. The CD86 molecules expression as well as the toll-like 4 receptor on dendritic cells was increased after oral administration of Lb. In addition, Lb attenuated the eosinophils infiltration, mucus production and the ovalbumin-specific IgE concentration in serum. Moreover, Lb inhibited the secretion of asthma-related inflammatory molecules such as IL-4, IL-5, IL-13 and eotaxin in bronchoalveolar lavage fluid (BALF). On the contrary, the IL-10 level in BALF was reduced by Lb. The analysis of lung tissue using real-time PCR revealed that Lb inhibited the local transcription of asthma-associated genes such as GATA3 and STAT6, and the otherwise Lb increased the expression of T-bet. The results contribute to the understanding of the probiotic modulator activities for the control of allergic diseases.
Footnotes
Cite this article as: European Respiratory Journal 2018 52: Suppl. 62, PA1107.
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- Copyright ©the authors 2018